Nitrogen Use Efficiency in Tea Plants: Mechanisms and Cultivar Screening Indicators

KONG Weiqi; WANG Liubin; XU Huan; WANG Liyuan; WEI Kang

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Journal of Tea Science ›› 2026, Vol. 46 ›› Issue (3) : 394-410.

Review

Nitrogen Use Efficiency in Tea Plants: Mechanisms and Cultivar Screening Indicators

KONG Weiqi^1,2, WANG Liubin¹, XU Huan³, WANG Liyuan^1,*, WEI Kang^1,*

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Abstract

Nitrogen (N) is an essential macronutrient for plant growth and development, and has been the subject of significant research. In recent years, breeding plants with high nitrogen use efficiency (NUE) has become a prominent focus, both theoretically and practically. However, compared with the remarkable research progress achieved in staple food crops such as rice and wheat, little systematic research has been conducted into cash crops. Tea plant (Camellia sinensis) is an important cash crop, with its economic benefits primarily derived from vegetative organs (buds and leaves). Nitrogen plays a crucial role in the growth, morphology, and quality formation of tea leaves. This paper reviewed the effects of nitrogen on tea plant growth and quality, summarized research progress on nitrogen-efficient tea plants from the perspectives of physiological processes, molecular regulatory mechanisms, and genetic foundations, outlined screening criteria for nitrogen-efficient tea cultivars, and provided future research directions.

Key words

tea plant / nitrogen-efficient mechanism / nitrogen use efficiency / cultivar screening

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KONG Weiqi, WANG Liubin, XU Huan, WANG Liyuan, WEI Kang. Nitrogen Use Efficiency in Tea Plants: Mechanisms and Cultivar Screening Indicators[J]. Journal of Tea Science. 2026, 46(3): 394-410

References

[1] 赵虎, 王海斌, 陈晓婷, 等. 茶树根际土壤氮素组成及其吸收利用效率分析[J]. 中国农业科技导报, 2020, 22(7): 148-154.
Zhao H, Wang H B, Chen X T, et al.Analysis of nitrogen composition and its absorption and utilization efficiency in rhizosphere soil of tea tree[J]. Journal of Agricultural Science and Technology, 2020, 22(7): 148-154.
[2] 马畅, 李旭, 吕小红, 等. 盐粳系列水稻氮肥效率差异及氮高效品种筛选[J]. 江苏农业科学, 2022, 50(13): 32-38.
Ma C, Li X, Lü X H, et al.Differences in nitrogen use efficiency among rice varieties of Yanjing series and screening of nitrogen-efficient varieties[J]. Jiangsu Agricultural Sciences, 2022, 50(13): 32-38.
[3] Liu Y Q, Wang H R, Jiang Z M, et al.Author correction: genomic basis of geographical adaptation to soil nitrogen in rice[J]. Nature, 2022, 610(7931): E4-E4.
[4] 宣伟, 徐国华. 植物适应土壤氮素环境的基因选择: 以水稻为例[J]. 植物学报, 2021, 56(1): 1-5.
Xuan W, Xu G H.Genomic basis of rice adaptation to soil nitrogen status[J]. Chinese Bulletin of Botany, 2021, 56(1): 1-5.
[5] Ruan L, Wang L Y, Wei K, et al.Comparative analysis of nitrogen spatial heterogeneity responses in low nitrogen susceptible and tolerant tea plants (Camellia sinensis)[J]. Scientia Horticulturae, 2019, 246: 182-189. https://doi.org/10.1016/j.scienta.2018.10.063.
[6] 廖金梅, 黄维, 李安生, 等. 氮素对茶树生长及茶园土壤环境的影响研究进展[J]. 中国农学通报, 2023, 39(16): 82-87.
Liao J M, Huang W, Li A S, et al.Effects of nitrogen on the growth of tea trees and soil environment in tea gardens: research progress[J]. Chinese Agricultural Science Bulletin, 2023, 39(16): 82-87.
[7] Ruan L, Wei K, Wang L Y, et al. Characteristics of NH₄+ and NO₃-fluxes in tea (Camellia sinensis) roots measured by scanning ion-selective electrode technique[J]. Scientific Reports (Nature Publisher Group), 2016, 6: 38370. https://doi.org/10.1038/srep38370.
[8] Ruan J Y, Gerendás J, Härdter R, et al.Effect of nitrogen form and root-zone pH on growth and nitrogen uptake of tea (Camellia sinensis) plants[J]. Annals of Botany, 2007, 99(2): 301-310.
[9] 黄双杰, 曹梦珍, 陈凌芝, 等. 氮素胁迫条件下茶树根系发育及生长素的响应[J]. 江苏农业学报, 2023, 39(3): 814-821.
Huang S J, Cao M Z, Chen L Z, et al.Auxin response and tea plant roots formation regulated by nitrogen stress[J]. Jiangsu Journal of Agricultural Sciences, 2023, 39(3): 814-821.
[10] Li A A, Xu C, Rogers A, et al.A global scale mechanistic model of photosynthetic capacity (LUNA V1.0)[J]. Geoscientific Model Development, 2016, 9(2): 587-606.
[11] Onoda Y, Wright I J, Evans J R, et al.Physiological and structural tradeoffs underlying the leaf economics spectrum[J]. The New Phytologist, 2017, 214(4): 1447-1463.
[12] Kouki H, Aki S.The role of Rubisco and cell walls in the interspecific variation in photosynthetic capacity[J]. Oecologia, 2009, 160(3): 443-451.
[13] Onoda Y, Hikosaka K, Hirose T.Allocation of nitrogen to cell walls decreases photosynthetic nitrogen-use efficiency[J]. Functional Ecology, 2004, 18(3): 419-425.
[14] Bloom A.The increased importance of distinguishing among plant nitrogen sources[J]. Current Opinion in Plant Biology, 2015, 25(1): 10-16.
[15] Feng L, Gao M J, Hou R Y, et al.Determination of quality constituents in the young leaves of albino tea cultivars[J]. Food Chemistry, 2014, 155: 98-104. https://doi.org/10.1016/j.foodchem.2014.01.044.
[16] 申瑞寒, 马立锋, 杨向德, 等. 氮素形态和弱光胁迫对茶树生长代谢的影响[J]. 茶叶科学, 2023, 43(3): 349-355.
Shen R H, Ma L F, Yang X D, et al.Effects of nitrogen form and weak light stress on tea plant growth and metabolism[J]. Journal of Tea Science, 2023, 43(3): 349-355.
[17] Li K, Liu D N, Li L Y, et al.Effects of nitrogen application amount on nitrogen distribution and photosynthesis in tea leaves[J]. Frontiers in Plant Science, 2025, 16: 1575317. https://doi.org/10.3389/fpls.2025.1575317.
[18] Zhang W J, Ni K, Long L Z, et al.Nitrogen transport and assimilation in tea plant (Camellia sinensis): a review[J]. Frontiers in Plant Science, 2023, 14: 1249202. https://doi.org/10.3389/fpls.2023.1249202.
[19] Liu Z W, Li H, Liu J X, et al. Integrative transcriptome, proteome,microRNA analysis reveals the effects of nitrogen sufficiency and deficiency conditions on theanine metabolism in the tea plant (Camellia sinensis)[J]. Horticulture Research, 2020, 7(1): 65. https://doi.org/10.1038/s41438-020-0290-8.
[20] Wang Y, Ouyang J X, Fan D M, et al.Transcriptome analysis of tea (Camellia sinensis) leaves in response to ammonium starvation and recovery[J]. Frontiers in Plant Science, 2022,(13): 963269. https://doi.org/10.3389/fpls.2022.963269.
[21] Ruan L, Wei K, Wang L Y, et al. Characteristics of free amino acids (the quality chemical components of tea) under spatial heterogeneity of different nitrogen forms in tea (Camellia sinensis) plants[J]. Molecules, 2019, 24(3): 415. https://doi.org/10.3390/molecules24030415.
[22] 邓敏, 徐泽, 胡留杰, 等. 不同氮营养水平对福鼎大白茶产量及品质的影响[J]. 西南农业学报, 2012, 25(4): 1330-1333.
Deng M, Xu Z, Hu L J, et al.Effect of different levels of nitrogen application on yield and quality of Fuding Dabaicha[J]. Southwest China Journal of Agricultural Sciences, 2012, 25(4): 1330-1333.
[23] 韩二玲, 秦永龙, 王兴飘, 等. 氮肥用量对‘白叶一号’茶树叶片碳氮代谢的调控[J]. 植物营养与肥料学报, 2025, 31(9): 1819-1829.
Han E L, Qin Y L, Wang X P, et al.Regulation of nitrogen fertilizer application rates on carbon and nitrogenmetabolism in leaves of ‘Baiye 1’ tea plants[J]. Journal of Plant Nutrition and Fertilizer Science, 2025, 31(9): 1819-1829.
[24] Chen Y C, Wang F, Wu Z D, et al. Effects of long-term nitrogen fertilization on the formation of metabolites related to tea quality in subtropical China[J]. Metabolites, 2021, 11(3): 146. https://doi.org/10.3390/metabo11030146.
[25] 刘美雅, 汤丹丹, 矫子昕, 等. 适宜氮肥施用量显著提升夏季绿茶品质[J]. 植物营养与肥料学报, 2021, 27(8): 1407-1419.
Liu M Y, Tang D D, Jiao Z X, et al.Suitable nitrogen fertilization rate effectively improve the quality of summer green tea[J]. Journal of Plant Nutrition and Fertilizer, 2021, 27(8): 1407-1419.
[26] Ruan J, Haerdter R, Gerendás J.Impact of nitrogen supply on carbon/nitrogen allocation: a case study on amino acids and catechins in green tea[Camellia sinensis (L.) O. Kuntze] plants[J]. Plant Biology, 2010, 12(5): 724-734.
[27] Li A S, Qiu Z H, Liao J M, et al. The effects of nitrogen fertilizer on the aroma of fresh tea leaves from Camellia sinensis cv. Jinxuan in summer and autumn[J]. Foods, 2024, 13(11): 1776. https://doi.org/10.3390/foods13111776.
[28] Liu M Y, Asdrubal B, Ma L F, et al. Lipidomics analysis unravels the effect of nitrogen fertilization on lipid metabolism in tea plant (Camellia sinensis L.)[J]. BMC Plant Biology, 2017, 17(1): 165. https://doi.org/10.1186/s12870-017-1111-6.
[29] Lin Z H, Chen C S, Zhong Q S, et al. The GC-TOF/MS-based metabolomic analysis reveals altered metabolic profiles in nitrogen-deficient leaves and roots of tea plants (Camellia sinensis)[J]. BMC Plant Biology, 2021, 21(1): 506. https://doi.org/10.1186/S12870-021-03285-Y.
[30] Zhu, Y, Lü H, Shao C, et al. Identification of key odorants responsible for chestnut-like aroma quality of green teas[J]. Food Research International, 2018, 108: 74-82. https://doi.org/10.1016/j.foodres.2018.03.026.
[31] Lin Z H, Zhong Q S, Chen C S, et al. Carbon dioxide assimilation and photosynthetic electron transport of tea leaves under nitrogen deficiency[J]. Botanical Studies, 2016, 57(1): 37. https://doi.org/10.1186/S40529-016-0152-8.
[32] 林郑和, 钟秋生, 郝志龙, 等. 低氮对不同茶树品种叶绿素荧光特性的影响[J]. 茶叶科学, 2017, 37(4): 363-372.
Lin Z H, Zhong Q S, Hao Z L, et al.Effects of chlorophyll fluorescence parameters of different tea cultivars in response to low nitrogen[J]. Journal of Tea Science, 2017, 37(4): 363-372.
[33] 詹少芸, 曹藩荣. 影响紫芽茶花青素含量的因素及其在茶树优质高产上的应用[J]. 广东茶业, 2019(4): 19-22.
Zhan S Y, Cao F R.Factors affecting anthocyanin content in purple bud tea and its application in high-quality and high-yield tea plants[J]. Guangdong Tea Industry, 2019(4): 19-22.
[34] 周慧, 张凯凯, 张欢, 等. 茶树花青素的代谢调控研究进展[J]. 食品安全质量检测学报, 2025, 16(3): 106-114.
Zhou H, Zhang K K, Zhang H, et al.Research progress on the regulation of anthocyanidins metabolism in tea plant[J]. Journal of Food Safety and Quality, 2025, 16(3): 106-114.
[35] 刘健伟. 基于组学技术研究氮素对于茶树碳氮代谢及主要品质成分生物合成的影响[D]. 北京: 中国农业科学院, 2016: 39-42.
Liu J W.Omics-based study on the metabolism of C/N and biosynthesis of main quality related components in tea plants affected by nitrogen[D]. Beijing: Chinese Academy of Agricultural Sciences, 2016: 39-42.
[36] 黄双杰, 李梦真, 罗金蕾, 等. 茶树根系发育及生长素对不同氮形态的响应[J]. 河南农业科学, 2024, 53(7): 54-65.
Huang S J, Li M Z, Luo J L, et al.Response of root development and auxin in tea plants to different nitrogen forms[J]. Journal of Henan Agricultural Sciences, 2024, 53(7): 54-65.
[37] Hu S K, Zhang M, Yang Y Q, et al. A novel insight into nitrogen and auxin signaling in lateral root formation in tea plant[Camellia sinensis (L.) O. Kuntze] [J]. BMC Plant Biology, 2020, 20(1): 232. https://doi.org/10.1186/s12870-020-02448-7.
[38] 宋博. 茶树CsAMT2s基因对铵态氮吸收调控机制研究[D]. 福州: 福建农林大学, 2023: 41-44.
Song B.Study on the mechanism of regulation of ammonium nitrogen uptake by tea plant CsAMT2s genes[D]. Fuzhou: Fujian Agriculture and Forestry University, 2023: 41-44.
[39] 张鹏, 张然然, 都韶婷. 植物体对硝态氮的吸收转运机制研究进展[J]. 植物营养与肥料学报, 2015, 21(3): 752-762.
Zhang P, Zhang R R, Du S T.Research advances in nitrate uptake and transport in plants[J]. Journal of Plant Nutrition and Fertilizer, 2015, 21(3): 752-762.
[40] 郑冬超. 调节转运蛋白AtNRT1.1、AtNRT2.1及PdAMT1.1基因表达对拟南芥和杨树氮素吸收的影响研究[D]. 北京: 北京林业大学, 2013: 2-3.
Zheng D C.Mediating the expression of AtNRT1.1, AtNRT2.1 and PdAMT1.1 genes affects nitrogen absorption in arabidopsis and poplar[D]. Beijing: Beijing Forestry University, 2013: 2-3.
[41] Léran S, Varala K, Boyer J C, et al.A unified nomenclature of NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER family members in plants[J]. Trends in Plant Science, 2014, 19(1): 5-9.
[42] 胡振民, 万青, 李欢, 等. 茶树CsNRT1.1基因密码子使用特性分析[J]. 江苏农业学报, 2019, 35(4): 896-903.
Hu Z M, Wan Q, Li H, et al.Analysis of codon usage features of CsNRT1.1 gene in Camellia sinensis[J]. Jiangsu Journal of Agricultural Sciences, 2019, 35(4): 896-903.
[43] 俸云燕, 周思如, 左同鸿, 等. 烟草NPF基因家族的全基因组鉴定和表达分析[J/OL]. 分子植物育种, 2023-11-22
[2026-04-12]. https://link.cnki.net/urlid/46.1068. S.20231121.1559.008. Feng Y Y, Zhou S R, Zuo T H, et al. Genome-Wide identification and expression analysis of tobacco NPF gene family[J/OL]. Molecular Plant Breeding, 2023-11-22[2026-04-12]. https://link.cnki.net/urlid/46.1068.S.20231121.1559.008.
[44] 袁青云, 韩昱, 贺巍, 等. 茶树CsNPF6.1/6.3基因克隆及ABA转运功能验证[J]. 园艺学报, 2024, 51(12): 2817-2828.
Yuan Q Y, Han Y, He W, et al.Cloning and ABA transport function analysis of CsNPF6.1/6.3 in tea plants[J]. Acta Horticulturae Sinica, 2024, 51(12): 2817-2828.
[45] 汪进, 添先凤, 江昌俊, 等. 茶树硝酸盐转运蛋白基因的克隆和表达分析[J]. 植物生理学报, 2014, 50(7): 983-988.
Wang J, Tian X F, Jiang C J, et al.Cloning and expression analysis of nitrate transporter gene in Camellia sinensis[J]. Plant Physiology Journal, 2014, 50(7): 983-988.
[46] 罗勇. 烟草高亲和硝酸盐转运蛋白基因NtNRT2.5的功能解析[D]. 郑州: 河南农业大学, 2021: 34-36.
Luo Y.Functional analysis of tobacco high-affinity nitrate transporter gene NtNRT2.5[D]. Zhengzhou: Henan Agricultural University, 2021: 34-36.
[47] 张文婧, 林琳, 孙威江. 茶树铵转运蛋白AMT研究进展[J]. 分子植物育种, 2022, 20(8): 2586-2596.
Zhang W J, Lin L, Sun W J.Advances of ammonium transporter AMT in tea plant (Camellia sinensis (L.) O. Kuntze.)[J]. Molecular Plant Breeding, 2022, 20(8): 2586-2596.
[48] 郝东利, 杨顺瑛, 黄亚楠, 等. 拟南芥铵转运蛋白AtAMT1.3的电生理功能[J]. 江苏农业科学, 2017, 45(8): 36-40.
Hao D L, Yang S Y, Huang Y N, et al.Electrophy siological function of ammonium transporter AtAMT1.3 in Arabidopsis thaliana[J]. Jiangsu Agricultural Sciences, 2017, 45(8): 36-40.
[49] 宋博, 张灵枝, 陈焯彤, 等. 不同施肥处理对盆栽不同品种茶树根系生理活性及CsAMT2s基因表达的影响[J]. 应用与环境生物学报, 2023, 29(5): 1253-1260.
Song B, Zhang L Z, Chen Z T, et al.Effects of different fertilization treatments on the physiological activities of rootsand the expression of CsAMT2s in potted tea cuttings of different varieties[J]. Chinese Journal of Applied and Environmental Biology, 2023, 29(5): 1253-1260.
[50] Motasim A M, Samsuri A W, Nabayi A, et al. Urea application in soil: processes, losses,alternatives: a review[J]. Discover Agriculture, 2024, 2(1): 42. https://doi.org/10.1007/s44279-024-00060-z.
[51] Liu M Y, Tang D D, Shi Y Z, et al.Foliar N application on tea plant at its dormancy stage increases the N concentration of mature leaves and improves the quality and yield of spring tea[J]. Frontiers in Plant Science, 2021, 12: 753086. https://doi.org/10.3389/FPLS.2021.753086.
[52] 周碧青, 陈成榕, 杨文浩, 等. 茶树对可溶性有机和无机态氮的吸收与运转特性[J]. 植物营养与肥料学报, 2017, 23(1): 189-195.
Zhou B Q, Chen C R, Yang W H, et al.Uptake and transport characteristics of soluble organic and inorganicnitrogen by tea plant.[J] Journal of Plant Nutrition and Fertilizers, 2017, 23(1): 189-195.
[53] Li F, Dong C X, Yang T Y, et al. The tea plant CsLHT1 and CsLHT6 transporters take up amino acids, as a nitrogen source, from the soil of organic tea plantations[J]. Horticulture Research, 2021, 8(1): 178. https://doi.org/10.1038/S41438-021-00615-X.
[54] 郭玲玲, 张芬, 张亚真, 等. 茶树CsLHTs亚家族基因的克隆与表达分析[J]. 茶叶科学, 2019, 39(3): 280-288.
Guo L L, Zhang F, Zhang Y Z, et al.Molecular cloning and expression analysis of CsLHTs gene subfamily in tea plants (Camellia sinensis)[J]. Journal of Tea Science, 2019, 39(3): 280-288.
[55] 徐洪超, 商靖, 刘铭荟, 等. 氮代谢相关酶的研究进展[J]. 安徽农业科学, 2022, 50(4): 17-20.
Xu H C, Shang J, Liu M H, et al.Research progress of enzymes related to nitrogen metabolism[J]. Journal of Anhui Agricultural Sciences, 2022, 50(4): 17-20.
[56] Yang T Y, Li H P, Tian Y L, et al. Transcriptional regulation of amino acid metabolism in response to nitrogen deficiency and nitrogen forms in tea plant root (Camellia sinensis L. O. Kuntze)[J]. Scientific Reports, 2020, 10(1): 6868. https://doi.org/10.1038/s41598-020-63835-6.
[57] 王羽. 茶树铵转运蛋白CsAMT1.3基因的功能分析[D]. 杭州: 浙江大学, 2022: 44-46.
Wang Y.Function analysis of ammonium transporter CsAMT1.3 gene in tea plants[D]. Hangzhou: Zhejiang University, 2022: 44-46.
[58] 韩莹. 氮磷钾配施对茶树生理代谢和茶叶品质的影响[D]. 郑州: 河南农业大学, 2011: 35-36.
Han Y.Theeffect of nitrogen phosphate and potassium proportion fertilizers on tea tree physiological metabolism and tea quality[D]. Zhengzhou: Henan Agricultural University, 2011: 35-36.
[59] 胡国策, 蒋家月, 田坤红, 等. 氮素形态和水平对茶树生理特性的影响[J]. 安徽农业大学学报, 2018, 45(4): 588-593.
Hu G C, Jiang J Y, Tian K H, et al.Effects of nitrogen forms and nitrogen levels on the physiological characteristics of tea plants[J]. Journal of Anhui Agricultural University, 2018, 45(4): 588-593.
[60] Li F, Li H P, Dong C X, et al.Theanine transporters are involved in nitrogen deficiency response in tea plant (Camellia sinensis L.)[J]. Plant Signaling & Behavior, 2020, 15(3): 1728109. https://doi.org/ 10.1080/15592324.2020.1728109.
[61] 郭玲玲, 张芬, 成浩, 等. 茶树CsAAPs亚家族基因的克隆与表达分析[J]. 茶叶科学, 2020, 40(4): 454-464.
Guo L L, Zhang F, Cheng H, et al.Molecular cloning and expression analysis of CsAAPs gene subfamily in Camellia Sinensis[J]. Journal of Tea Science, 2020, 40(4): 454-464.
[62] Liu M Y, Jiao Z X, Lou H Q, et al. A glutamine synthetase-Dof transcription factor module regulates nitrogen remobilization from source to sink tissues in tea plants[J]. Plant Physiology, 2024, 197(1): kiae644. https://doi.org/10.1093/PLPHYS/KIAE644.
[63] 夏侯龙, 马丹妮, 张乐慧, 等. 过表达茶树自噬相关基因CsATG3b对拟南芥氮利用效率的影响[J]. 华中农业大学学报, 2023, 42(6): 127-137.
Xia H L, Ma D N, Zhang L H, et al.Effect of overexpressing tea plant autophagy-related gene CsATG3b on nitrogen use efficiency of Arabidopsis thaliana[J]. Journal of Huazhong Agricultural University, 2023, 42(6): 127-137.
[64] 黄玮. 茶树氨基酸转运基因CsLHTs及自噬相关基因CsATGs氮素利用功能解析[D]. 武汉: 华中农业大学, 2022: 148-150.
Huang W.Characterization of tea plant amino acid transporters CsLHTs and autophagy related genes CsATGs involved innitrogen utilization[D]. Wuhan: Huazhong Agricultural University, 2022: 148-150.
[65] Njogu R E, Kariuki D, Kamau D M, et al.Effects of foliar fertilizer application on quality of tea (Camellia sinensis) grown in the Kenyan highlands[J]. American Journal of Plant Sciences, 2014, 5(18): 2707-2715.
[66] 李静, 夏建国. 氮磷钾与茶叶品质关系的研究综述[J]. 中国农学通报, 2005(1): 62-65, 75.
Li J, Xia J G.Summary on nitrogen (N), phosphorus (P), potassium (K) and tea quality[J]. Chinese Agricultural Science Bulletin, 2005(1): 62-65, 75.
[67] Zhang Q F, Shi Y T, Hu H, et al.Magnesium promotes tea plant growth via enhanced glutamine synthetase-mediated nitrogen assimilation[J]. Plant Physiology, 2023, 192(2): 1321-1337.
[68] Xu W L, Li R, Zhang X Y, et al.Zinc/Iron-Regulated transporter-like protein CsZIP4 enhances zinc and nitrogen uptake and alleviates zinc stresses with nitrogen supply in Camellia sinensis[J]. Journal of Agricultural and Food Chemistry, 2024, 72(38): 21193-21207.
[69] Zhao S X, Bao Y L, Jin Z, et al. Effects of the combined application of nitrogen and selenium on tea quality and the expression of genes involved in nitrogen uptake and utilization in tea cultivar ‘Chuancha No.2’[J]. Agronomy, 2023, 13(12): 2997. https://doi.org/10.3390/AGRONOMY13122997.
[70] 李敬. 酸沉降加重对茶树铝根际过程和茶园铝分布格局的影响[D]. 南昌: 南昌大学, 2013: 35-36, 50.
Li J.Effects of elevated acid deposition on Al rhizosphere process and Al distribution pattern in tea plantation[D]. Nanchang: Nanchang University, 2013: 35-36, 50.
[71] Zhang W J, Dong X Y, Ni K, et al. Characterization of nitrate use efficiency in tea plant (Camellia sinensis) based on leaf chlorate sensitivity[J]. Horticulture Research, 2025, 12(4): uhae354. https://doi.org/10.1093/HR/UHAE354.
[72] 向芬, 李维, 刘红艳, 等. 氮素水平对茶树叶片氮代谢关键酶活性及非结构性碳水化合物的影响[J]. 生态学报, 2019, 39(24): 9052-9057.
Xiang F, Li W, Liu H Y, et al.Effects of nitrogen levels on key enzyme activities and non-structural carbohydratesin nitrogen metabolism in tea leaves[J]. Acta Ecologica Sinica, 2019, 39(24): 9052-9057.
[73] Huang H, Yao Q Y, Xia E H, et al.Metabolomics and transcriptomics analyses reveal nitrogen influences on the accumulation of flavonoids and amino acids in young shoots of tea plant (Camellia sinensis L.) associated with tea flavor[J]. Journal of Agricultural and Food Chemistry, 2018, 66(37): 9828-9838.
[74] Chen Z P, Li H P, Yang T Y, et al.Transcriptome analysis provides insights into the molecular bases in response to different nitrogen forms-induced oxidative stress in tea plant roots (Camellia sinensis)[J]. Functional Plant Biology, 2020, 47(12): 1073-1082.
[75] Zhang F, Liu Y, Wang L Y, et al.Molecular cloning and expression analysis of ammonium transporters in tea plants (Camellia sinensis (L.) O. Kuntze) under different nitrogen treatments[J]. Gene, 2018, 658: 136-145. https://doi.org/10.1016/j.gene.2018.03.024.
[76] 潘科, 李琴, 方仕茂, 等. 贵州代表性茶树品种茶青萎凋期游离氨基酸动态分析及评价[J]. 食品与发酵工业, 2021, 47(8): 82-89.
Pan K, Li Q, Fang S M, et al.Dynamic analysis and evaluation of free amino acids of typical tea varieties in Guizhou during withering stage[J]. Food and Fermentation Industries, 2021, 47(8): 82-89.
[77] 李宏, 邱芬奇, 李友莲. 植物数量性状基因座(QTL)的作图与克隆[J]. 科技情报开发与经济, 2008(2): 145-147.
Li H, Qiu F Q, Li Y L.The mapping and cloning of QTL (Quantitative Trait Locus) of the plant[J]. Sci-Tech Information Development and Economy, 2008(2): 145-147.
[78] 张东阳. 茶树游离氨基酸与儿茶素含量的QTL定位及候选基因筛选[D]. 雅安: 四川农业大学, 2024: 27-36.
Zhang D Y.QTL mapping and identification of candidate genes for free amino acids and catechins in tea plants[D]. Yaan: Sichuan Agricultural University, 2024: 27-36.
[79] Huang R, Wang J Y, Yao M Z, et al. Quantitative trait loci mapping for free amino acid content using an albino population and SNP markers provides insight into the genetic improvement of tea plants[J]. Horticulture Research, 2022, 9: uhab029. doi: 10.1093/HR/UHAB029.
[80] Wei K, Wang X C, Hao X Y, et al.Development of a genome-wide 200K SNP array and its application for high-density genetic mapping and origin analysis of Camellia sinensis[J]. Plant Biotechnology Journal, 2021, 20(3): 414-416.
[81] 黄丹娟. 茶树铝含量QTL定位及候选基因鉴定[D]. 武汉: 华中农业大学, 2023: 11-39.
Huang D J.QTL mapping of aluminum content and identification of candidate genes in tea plant[D]. Wuhan: Huazhong Agricultural University, 2023: 11-39.
[82] 徐礼羿. 茶树SNP高密度遗传连锁图谱构建与数量性状候选基因挖掘[D]. 武汉: 华中农业大学, 2019: 42-48.
Xu L Y.Construction of high-density SNP genetic map and identification of candidate genes for quantitative traits in Camellia sinensis[D]. Wuhan: Huazhong Agricultural University, 2019: 42-48.
[83] 杨亦扬, 马立锋, 石元值, 等. 叶绿素仪(SPAD)在茶树氮素营养诊断中的适用性研究[J]. 茶叶科学, 2008, 28(4): 301-308.
Yang Y Y, Ma L F, Shi Y Z, et al.Evaluation of nitrogen status in tea plants by SPAD[J]. Journal of Tea Science, 2008, 28(4): 301-308.
[84] 王丽鸳, 陈常颂, 林郑和, 等. 不同品种茶树生长对氮素浓度的响应差异[J]. 茶叶科学, 2015, 35(5): 423-428.
Wang L Y, Chen C S, Lin Z H, et al.Growth characteristic of different cultivars of tea plantin response to nitrogen contents[J]. Journal of Tea Science, 2015, 35(5): 423-428.
[85] Zheng S, Ni K, Chai H, et al. Study on the nitrogen response and low nitrogen tolerance variations in different tea varieties[J]. Agronomy, 2025, 15(4): 815. https://doi.org/10.3390/AGRONOMY15040815.
[86] 王新超, 杨亚军, 陈亮, 等. 茶树氮素利用效率相关生理生化指标初探[J]. 作物学报, 2005(7): 926-931.
Wang X C, Yang Y J, Chen L, et al.Preliminary study on physiological and biochemical indices related to nitrogen use efficiency in tea plant(Camellia sinensis, 2005(7): 926-931.
[87] 王化敦, 张鹏, 马鸿翔. 植物氮素吸收利用相关NPF基因家族研究进展[J]. 植物营养与肥料学报, 2022, 28(8): 1520-1534.
Wang H D, Zhang P, Ma H X.Research advances on NPF gene families in plant nitrogen uptake and utilization[J]. Journal of Plant Nutrition and Fertilizer, 2022, 28(8): 1520-1534.
[88] 钱园凤, 杨高中, 徐伟林, 等. 基于代谢组学的崇觉罗汉茶主要化学成分分析[J]. 食品科学, 2025, 46(3): 138-145.
Qian Y F, Yang G Z, Xu W L, et al.Metabolomic analysis of the chemical composition of chongjue luohan tea[J]. Food Science, 2025, 46(3): 138-145.
[89] Kuzmina D, Malyukova L S, Manakhova K, et al. Associations between SNPs and vegetation indices: unraveling molecular insights for enhanced cultivation of tea plant (Camellia sinensis (L.) O. Kuntze)[J]. PeerJ, 2024, 12: e17689. https://doi.org/10.7717/PEERJ.17689.
[90] Fan K, Zhang J, Wang M, et al. Development and application of SNP-KASP markers based on genes related to nitrogen uptake, assimilation and allocation in tea plant (Camellia sinensis L.)[J]. Agronomy, 2022, 12(10): 2534. https://doi.org/10.3390/agronomy12102534.
[91] Ruan L, Wei K, Li J W, et al.Responses of tea plants (Camellia sinensis) with different low-nitrogen tolerances during recovery from nitrogen deficiency[J]. Journal of the Science of Food and Agriculture, 2022, 102(4): 1405-1414.