Study on the Composition and Content of Advanced Glycation End-Products in Different Teas

NIU Li, SUN Mufang, JIANG Yongwen, ZHU Hongkai

Journal of Tea Science ›› 2026, Vol. 46 ›› Issue (3) : 489-498.

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Journal of Tea Science ›› 2026, Vol. 46 ›› Issue (3) : 489-498.
Research Paper

Study on the Composition and Content of Advanced Glycation End-Products in Different Teas

  • NIU Li1, SUN Mufang2,*, JIANG Yongwen3, ZHU Hongkai3,*
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Abstract

To clarify the content and composition of advanced glycation end products (AGEs) in different types of tea, 13 AGEs derived from lysine and arginine in green tea, white tea, oolong tea, black tea, and dark tea were analyzed using ultra-high performance liquid chromatography-tandem mass spectrometry. The results indicate that 9 AGEs were detected in all tea samples, arginine-derived methylglyoxal-hydroimidazolones (MG-Hs) being the predominant. The total AGEs contents in different tea typesfollowed the trend of green tea=white tea>dark tea>black tea>oolong tea (P<0.05). A high catechin content in tea did not effectively inhibit the formation of AGEs. Instead, they might serve as one of the contributing factors to elevated AGEs. This study aimed to characterize the overall profile of AGEs composition in tea, providing a comprehensive evaluation of protein thermal damage during tea processing.

Key words

lysine / arginine / α-dicarbonyl / AGEs / furosine / Maillard reaction

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NIU Li, SUN Mufang, JIANG Yongwen, ZHU Hongkai. Study on the Composition and Content of Advanced Glycation End-Products in Different Teas[J]. Journal of Tea Science. 2026, 46(3): 489-498

References

[1] Ho C T, Zheng X, Li S M.Tea aroma formation[J]. Food Science and Human Wellness, 2015, 4(1): 9-27.
[2] Ruan D L, Wang H, Cheng F L.The Maillard reaction in food chemistry: current technology and applications[D]. Cham: Springer, 2019: 1-21.
[3] Uribarri J, Del Castillo M D, De la Maza M P, et al. Dietary advanced glycation end products and their role in health and disease[J]. Advances in Nutrition, 2015, 6(4): 461-473.
[4] Scheijen J L, Hanssen N M, Van Greevenbroek M M, et al. Dietary intake of advanced glycation endproducts is associated with higher levels of advanced glycation endproducts in plasma and urine: the CODAM study[J]. Clinical Nutrition, 2018, 37(3): 919-925.
[5] Vlassara H, Cai W, Crandall J, et al.Inflammatory mediators are induced by dietary glycotoxins, a major risk factor for diabetic angiopathy[J]. PNAS, 2002, 99(24): 15596-15601.
[6] Poojary M M, Zhang W, Greco I, et al.Liquid chromatography quadrupole-orbitrap mass spectrometry for the simultaneous analysis of advanced glycation end products and protein-derived cross-links in food and biological matrices[J]. Journal of Chromatography A, 2019: 460767. https://doi.org/10.1016/j.chroma.2019.460767.
[7] Mildner-Szkudlarz S, Siger A, Szwengiel A, et al.Phenolic compounds reduce formation of Nε-(carboxymethyl)lysine and pyrazines formed by Maillard reactions in a model bread system[J]. Food Chemistry, 2017, 231: 175-184. https://doi.org/10.1016/j.foodchem.2017.03.126.
[8] Jiao Y, He J L, Li F L, et al.Nε-(carboxymethyl)lysine and Nε-(carboxyethyl)lysine in tea and the factors affecting their formation[J]. Food Chemistry, 2017, 232: 683-688. doi: 10.1016/j.foodchem.2017.04.059.
[9] Horanni R, Engelhardt U H.Determination of amino acids in white, green, black, oolong, Pu-erh teas and tea products[J]. Journal of Food Composition and Analysis, 2013, 31(1): 94-100.
[10] Zhang W, Zhang B, Ye Y, et al.Methylglyoxal-hydroimidazolones (MG-Hs) instead of Nɛ-(carboxymethyl)-L-lysine (CML) is the major advanced glycation endproduct during drying process in black tea[J]. Food Chemistry, 2020, 333: 127499. https://doi.org/10.1016/j.foodchem.2020.127499.
[11] Yılmaz C, Özdemir F, Gökmen V.Investigation of free amino acids, bioactive and neuroactive compounds in different types of tea and effect of black tea processing[J]. LWT-Food Science and Technology, 2020, 117: 108655. https://doi.org/10.1016/j.lwt.2019.108655.
[12] Li M R, Zhai X T, Wan X C, et al.Formation of aroma active volatiles from thermal degradation of 18 amino acids with or without sugars in low-moisture systems and the modulation by sucrose and epigallocatechin gallate[J]. Food Research International, 2025, 222: 117618. https://doi.org/10.1016/j.foodres.2025.117618.
[13] Hwang H I, Hartman T G, Ho C T.Relative reactivities of pyridines, pyrroles, and oxazoles[J]. Journal of Agricultural and Food Chemistry, 1995, 43(11): 2917-2921.
[14] Zan J Z, Chen W X, Yuan H B, et al.Evaluation of key taste components in Huangjin green tea based on electronic tongue technology[J]. Food Research International, 2025, 201: 115569. https://doi.org/10.1016/j.foodres.2024.115569.
[15] Erbersdobler H F, Somoza V.Forty years of furosine-forty years of using Maillard reaction products as indicators of the nutritional quality of foods[J]. Molecular Nutrition & Food Research, 2007, 51(4): 423-430.
[16] Yu H, Zhong Q L, Xie Y F, et al.Kinetic study on the generation of furosine and pyrraline in a Maillard reaction model system of D-glucose and L-lysine[J]. Food Chemistry, 2020, 317: 126458. https://doi.org/10.1016/j.foodchem.2020.126458.
[17] 陈文雪, 诸力, 刘跃云, 等. α-二羰基化合物在不同茶类中的含量分布研究[J]. 茶叶科学, 2025, 45(4): 604-614.
Chen W X, Zhu L, Liu Y Y, ea al. Study on disrtibution of α-dicarbonyl in different teas[J]. Journal of Tea Science, 2025, 45(4): 604-614.
[18] Taş N G, Gökmen V.Effect of roasting and storage on the formation of Maillard reaction and sugar degradation products in hazelnuts (Corylus avellana L.)[J]. Journal of Agricultural and Food Chemistry, 2019, 67(1): 415-424.
[19] Berk E, Hamzalıoğlu A, Gökmen V.Investigations on the Maillard reaction in sesame (Sesamum indicum L.) seeds induced by roasting[J]. Journal of Agricultural and Food Chemistry, 2019, 67(17): 4923-4930.
[20] Shao X, Chen H D, Zhu Y D, et al.Essential structural requirements and additive effects for flavonoids to scavenge methylglyoxal[J]. Journal of Agricultural and Food Chemistry, 2014, 62(14): 3202-3210.
[21] Poojary M M, Zhang W, Olesen S B, et al.Greentea extract decreases Arg-derived advanced glycation endproducts but not Lys-derived ages in UHT milk during 1-year storage[J]. Journal of Agricultural and Food Chemistry, 2020, 68(48): 14261-14273.
[22] Nagai R, Araki T, Hayashi C M, et al.Identification of Nε-(carboxyethyl)lysine, one of the methylglyoxal-derived AGE structures, in glucose-modified protein: mechanism for protein modification by reactive aldehydes[J]. Journal of Chromatography B, 2003, 788(1): 75-84.
[23] Zhang J, Guo C, Cui X Y, et al.A two-level strategy for standardization of near infrared spectra by multi-level simultaneous component analysis[J]. Analytica Chimica Acta, 2019, 1050: 25-31. https://doi.org/10.1016/j.aca.2018.11.013.
[24] Zhu Y D, Snooks H, Sang S M.Complexity of advanced glycation end products in foods: where are we now?[J]. Journal of Agricultural and Food Chemistry, 2018, 66(6): 1325-1329.
[25] Zhang W, Poojary M M, Rauh V, et al.Quantitation of α-dicarbonyls and advanced glycation endproducts in conventional and lactose-hydrolyzed ultrahigh temperature milk during 1 year of storage[J]. Journal of Agricultural and Food Chemistry, 2019, 67(46): 12863-12874.
[26] Navarro M, Morales F J.Mechanism of reactive carbonyl species trapping by hydroxytyrosol under simulated physiological conditions[J]. Food Chemistry, 2015, 175: 92-99. https://doi.org/10.1016/j.foodchem.2014.11.117.
[27] Navarro M, Atzenbeck L, Pischetsrieder M, et al.Investigations on the reaction of C3 and C6 α-dicarbonyl compounds with hydroxytyrosol and related compounds under competitive conditions[J]. Journal of Agricultural and Food Chemistry, 2016, 64(32): 6327-6332.
[28] Klöpfer A, Spanneberg R, Glomb M A.Formation of arginine modifications in a model system of Nα-tert-butoxycarbonyl (Boc)-arginine with methylglyoxal[J]. Journal of Agricultural and Food Chemistry, 2011, 59(1): 394-401.
[29] Henle T.Protein-bound advanced glycation endproducts (AGEs) as bioactive amino acid derivatives in foods[J]. Amino Acids, 2005, 29(4): 313-322.
[30] Poulsen M W, Hedegaard R V, Andersen J M, et al.Advanced glycation endproducts in food and their effects on health[J]. Food and Chemical Toxicology, 2013, 60: 10-37. https://doi.org/10.1016/j.fct.2013.06.052.
[31] Li N, Taylor L S, Mauer L J.Degradation kinetics of catechins in green tea powder: effects of temperature and relativehumidity[J]. Journal of Agricultural and Food Chemistry, 2011, 59(11): 6082-6090.
[32] Nomi Y, Annaka H, Sato S, et al.Simultaneous quantitation of advanced glycation end products in soy sauce and beer by liquid chromatography-tandem mass spectrometry without ion-pair reagents and derivatization[J]. Journal of Agricultural and Food Chemistry, 2016, 64(44): 8397-8405.
[33] Lee H H L, Ha S K, Kim Y, et al. Simultaneous analysis of advanced glycation end products using dansyl derivatization[J]. Food Chemistry, 2024, 432: 137186. https://doi.org/10.1016/j.foodchem.2023.137186.
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