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Journal of Tea Science ›› 2014, Vol. 34 ›› Issue (5): 435-441.doi: 10.13305/j.cnki.jts.2014.05.003

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Research on Aspergillus flavus Toxin-producing in Pu'er Tea Fermentation

LI Yali1, KANG Guanhong1,2, YANG Liyuan3, ZHU Hongkun4, LI Zhiying3, ZHOU Hongjie1,*   

  1. 1. College of Pu'er tea, Yunnan Agriculture University , Kunming 650201, China;
    2. Yunnan Xiaguan Tuocha Co., Ltd., Dali 676700, China;
    3. Institute of Microbiology, Yunnan University, Kunming 650091, China;
    4. Yunnan Institute of Product Quality Supervision and Inspection, Kunming 650223, China
  • Received:2014-02-12 Revised:2014-03-05 Published:2019-09-03

Abstract: For the purpose of studying the Aspergillus flavus growth and toxin production in Pu′er tea fermentation, the Yunnan large-leaves solar-drying green tea was used as the raw material to fermentate by inoculating toxigenic Aspergillus flavus fungus in Pu′er tea. Aflatoxin was tested by LC-MS/MS method at the end of tea samples fermentation. The results showed that during the tea fermentation, Aspergillus flavus growth was observed in the initial fermentation. However, with the extension of Pu′er tea fermentation time, Aspergillus flavus growth were inhibited in the late fermentation, the number decreased gradually, while the end of fermentation, the aflatoxin was not detected in tea samples.

Key words: Pu'er tea, Aspergillus flavus, aflatoxins, LC-MS/MS

CLC Number: