Study on Variation of Chlorophyllase Activity in Tea Plant (Camellia sinensis)

LI Yuan-hua; GU Wei; NI De-jiang; YANG Jiang-fan; ZHENG Fang; SHI Yu-tao

doi:10.13305/j.cnki.jts.2011.01.005

PDF(176 KB)

Journal of Tea Science ›› 2011, Vol. 31 ›› Issue (1) : 27-32. DOI: 10.13305/j.cnki.jts.2011.01.005

Study on Variation of Chlorophyllase Activity in Tea Plant (Camellia sinensis)

LI Yuan-hua^1,2, GU Wei², NI De-jiang², YANG Jiang-fan¹, ZHENG Fang², SHI Yu-tao¹

Author information +

History +

Abstract

The variation of chlorophyllase activity in tea plant（Camellia Sinensis）was investigated. The results showed that the diurnal variation of chlorophyllase activity decreased gradually from 7:00 to 16:00, and then showed an upward tendency from 16:00 to 19:00. The chlorophyllase activity diversity also existed in various seasons and leaf tenderness. The activity of chlorophyllase in the shoots of tea plant was highest in spring and lowest in autumn, and it took second place in summer. In the different growth position of tea plant, the chlorophyllase activity decreased with an order of bud > the first leaf > the second leaf > the third leaf > the old leaf > the young stem. During the fresh leaf spreading process of 6-hour treatment, the activity of chlorophyllase declined at the beginning and increased greatly later. The activity of chlorophyllase in eight different cultivars of Camellia Sinensis was determined. The results indicated that the sequence of the chlorophyllase activity among different cultivars from high to low was Fuyunliuhao>Fudingdahao>Fudingdahao>Echa1>Pingyangtezao>Wuniuzao>Yingshuang>Longjing 43. Further than that, the influence of overhead shading in tea garden on the chlorophyllase activity was studied. The result suggested that the activity of chlorophyllase both decreased after the treatment of 60% shading rate and 80% shading rate.

Key words

chlorophyllase / enzyme activity / tea plant

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

LI Yuan-hua, GU Wei, NI De-jiang, YANG Jiang-fan, ZHENG Fang, SHI Yu-tao. Study on Variation of Chlorophyllase Activity in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science. 2011, 31(1): 27-32 https://doi.org/10.13305/j.cnki.jts.2011.01.005

References

[1] 沈成国, 张福锁, 毛达如. 植物叶片哀老过程中叶绿素降解代谢研究进展[J]. 植物学通报, 1998, 15(增刊): 41-46.
[2] Debora Jacob-Wilk, Doron Holland, Eliezer E, et al. Chlorophyll breakdown by chlorophyllase: isolation and functional expression of Chlase gene from ethylene-treated Citrus fruit and its regulation during development[J]. Plant Journal, 1999,20(6): 653-661.
[3] Fernandez-Lopez, J A Almela L, et al. Partial purification and properties of chlorophyllase from chlorotic Citrus limon leaves[J]. Photochemistry, 1992, 31(2): 447-449.
[4] Mcfeeters RF, Chichester CO, Whitaker JR.Purification and properties of chlorophyllase from Ailanthus altisstima(tree-of-heaven)[J]. Plant Physiol, 1971, 47(5): 609-618.
[5] Nagao Ogura.Studies on chlorophyllase in tea leave Ⅲ. Properties of soluble and insoluble chlorophyllase[J]. Plant and cell physiology, 1972, 13(6): 971-979.
[6] Mioko Kuroki, Yuzo Shioi, Tsutomu SASA.Purification and Properties of soluble chlorophyllase from tea leaf sprouts[J]. Plant and cell physiology, 1981, 22(4): 717-725.
[7] Tanaka K, Kakuno T, Yamashita J, et al. Purification and properties of chlorophyllase from greened rye seeding[J]. J Biochem, 1982, 92(6): 1763-1773.
[8] K R Hirschfeld, E E Goldschmidt.Chlorophyllase activity in Chlorophyll-free Citrus chloroplasts[J]. Plant Cell Reports, 1983, 2(3): 117-118.
[9] Rodoni S, Schellenberg M, Matile P.Chloroplast breakdown in senescing arley leaves as correlated with phaeophorbide an oxygenase activity[J]. Plant Physiol, 1998, 152(2/3): 139-144.
[10] Brandis A, Vainstein A, Goldschmidt E E.Distribution of chlorophyllase among components of chloroplast membranes in Citrus Sinensis organs[J]. Plant Physiology and Biochemistry, 1996, 34(1): 49-54.
[11] Matile P, Schellenberc M, Vicentini F.Localization of chlorophyllase in the chloroplast envelop[J]. Planta, 1997, 201(1): 96-99.
[12] Katsunori Kohata, Kouichi Hanada, Yuji Yamauchi, et al. Pheophorbide a content and chlorophyllase activity in green tea[J]. Biosci Biotechnol Biochem, 1998, 62(9): 1660-1663.
[13] 甘志军, 王晓云. 叶绿素酶的研究进展[J]. 生命科学研究, 2002, 6(1): 21-24.
[14] 李远华, 顾玮, 倪德江, 等. 茶树叶绿素酶提取、分离纯化与性质的初步研究[J]. 茶叶科学, 2009, 29(6): 412-418.
[15] Roger F, Mcfeeters. Substrate specificity of chlorophyllase[J]. Plant Physiology, 1975, 55(2): 377-381.
[16] AmirShapira D, Goldschmidt EE, AItman A. Chloropyhll catabolism in senescing plant tissue: In vivo breakdown intermediates suggest different derivative pathways for Citrus fruit and parsley leaves[J]. Proc Natl Accad Sci USA, 1987, 84(7): 1901-1905.
[17] 唐蕾. 银杏叶生长过程中的叶绿素酶活性变化[J]. 植物生理学通讯, 2002, 38(6): 561-563.
[18] Tsuchiya T, Ohta H.Cloning of chlorophyllase, a key enzyme in chlorophyll degradation: finding if a lipase motif and the induction by methyl jasmonate[J]. Proc Natl Acad Sci USA, 1999, 96(26): 15362-15367.
[19] 宛晓春. 茶叶生物化学[M]. 北京:中国农业出版社, 2003: 234-235.