Molecular Cloning and Expression of a Flowering-related Calcium-dependent Protein Kinase Gene in Tea Plant [Camellia sinensis (L.)O.Kuntze]

CHEN Xuan; TANG Cha-qin; ZOU Zhong-wei; YANG Yi-yang; FANG Wan-ping; WANG Yu-hua; LI Xing-hui

doi:10.13305/j.cnki.jts.2009.1.008

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Journal of Tea Science ›› 2009, Vol. 29 ›› Issue (1) : 47-52. DOI: 10.13305/j.cnki.jts.2009.1.008

Molecular Cloning and Expression of a Flowering-related Calcium-dependent Protein Kinase Gene in Tea Plant [Camellia sinensis (L.)O.Kuntze]

CHEN Xuan¹, TANG Cha-qin^1,2, ZOU Zhong-wei¹, YANG Yi-yang¹, FANG Wan-ping^1,*, WANG Yu-hua¹, LI Xing-hui^1,*

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Abstract

To have an insight into the comprehensive molecular characteristics relating to the flower development mechanisms of tea plant [Camellia sinensis (L.)O.Kuntze], cDNA-AFLP (Amplified Fragment Length Polymorphism) was performed within two tea plant cultivars, Longjin43 and Daye Wulong. A cDNA fragment encoding a calcium-dependent protein kinase gene was isolated and identified. The complete cDNA sequence was cloned by RACE (rapid amplification of cDNA ends), named TCK(Camellia sinensis calcium-dependent protein kinase)gene. GenBank accession number is EU732607. To identify differential expression of TCK gene, RT-PCR experiments were performed in six samples including leaves, small flower buds and big flower buds of Longjing 43 and Daye Wulong. Results showed that the expect band exists in the big flower buds, no band in leaves and small flower buds of these two cultivars. It hinted that TCK might play a key role during flower development of tea plant.

Key words

Camellia sinensis / calcium-dependent protein kinase gene / cloning / differential expression / flower development

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CHEN Xuan, TANG Cha-qin, ZOU Zhong-wei, YANG Yi-yang, FANG Wan-ping, WANG Yu-hua, LI Xing-hui. Molecular Cloning and Expression of a Flowering-related Calcium-dependent Protein Kinase Gene in Tea Plant [Camellia sinensis (L.)O.Kuntze][J]. Journal of Tea Science. 2009, 29(1): 47-52 https://doi.org/10.13305/j.cnki.jts.2009.1.008

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