Isolation and Molecular Identification of Fungal Colonization of Pu-erh Tea

ZHAO Zhen-jun; TONG Hua-rong; ZHOU Li; LIU Qin-jin

doi:10.13305/j.cnki.jts.2009.6.06

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Journal of Tea Science ›› 2009, Vol. 29 ›› Issue (6) : 436-442. DOI: 10.13305/j.cnki.jts.2009.6.06

Isolation and Molecular Identification of Fungal Colonization of Pu-erh Tea

ZHAO Zhen-jun¹, TONG Hua-rong², ZHOU Li², LIU Qin-jin^2,*

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Abstract

The fungal colonization was investigated from 60 representative samples by using the method of dilution plating on the medium of Dichloran Rose-Bengal Chloramphenicol Agar (DRBC) and dichloran 18% glycerol (DG18) agar. The results of the survey showed that the fungal colonization ranged from 1.6×10³ to 1.16×10⁵ cfu/g, and 71 isolates were identified by means of sequencing the rDNA fragments, which belong to 40 species of 19 genera, including 13 species of Aspergillus and 7 species of Penicillium, and 20 species of other genera. The predominant fungi in number were yeasts and different species of the genus Aspergillus. Further evaluation on the safety of these fungi isolated from Pu-erh tea products is needed.

Key words

fungal colonization / molecular identification / Pu-erh tea

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ZHAO Zhen-jun, TONG Hua-rong, ZHOU Li, LIU Qin-jin. Isolation and Molecular Identification of Fungal Colonization of Pu-erh Tea[J]. Journal of Tea Science. 2009, 29(6): 436-442 https://doi.org/10.13305/j.cnki.jts.2009.6.06

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