儿茶素组成和理化条件对茶黄素酶催化合成的影响

doi:10.13305/j.cnki.jts.2007.03.010

Abstract

Abstract: An in vitro model fermentation system, containing tea catechins and crude pear polyphenol oxidase from pear fruits has been used to determine the effect of tea catechin mixtures of different proportions & physico-chemical conditions on the formation of theaflavin. The optimum condition of theaflavins is 30℃ and The pH optima for theaflavin formation were 5.5. The tea catechins(EGC>200βmg/g, EGCG>200βmg/g, total catechins>500βmg/g) concentration is 5βmg/ml and the concentration of enzyme is 75βml/1β000βmg. pH and temperature in the model fermentation system are the most important factors (P<0.05).

Key words: theaflavin, proportions of tea catechin, physical and chemical conditions, enzymatic synthesis

CLC Number:

S571.1
Q523

WANG Kun-bo, LIU Zhong-hua, ZHAO Shu-juan, FU Dong-he, HUANG Jian-an. Effects of Tea Catechin Proportions & Physico-chemical Conditions on the Enzymatic Synthesis of Theaflavin[J]. Journal of Tea Science, 2007, 27(3): 192-200.

References

[1] 李立祥, 萧伟祥. 茶色素及茶黄素药理作用研究进展[J]. 福建茶叶, 2002(4): 35~37.
[2] Zhen Yong-su,Chen Zongmao, Cheng Shu-jun et al. Tea bioactivity and therapeutic potential[M]. Taylor & Francis, CRC press Inc.,2002.
[3] 李立祥, 萧伟祥. 茶黄素制取与分离研究进展[J]. 中国茶叶加工, 2000(4): 29~33．
[4] 萧伟祥, 钟瑾, 胡耀武, 等. 双液相系统酶化学技术制取茶色素[J]. 天然产物研究与开发, 2001, 13(5): 49~52.
[5] Takashi Tanaka, chie mine, kyoko inoue, et al. Synthesis of Theaflavin from Epicatechin and Epigallocatechin by Plant Homogenates and Role of Epicatechin Quinone in the Synthesis and Degradation of Theaflavin[J]. J. Agric. Food Chem. 2002, 50, 2142~2148.
[6] Shengmin Sang, Joshua D. Lambert, Shiying Tian et al. Enzymatic synthesis of tea theaflavin derivatives and theiranti-inflammatory and cytotoxic activities [J]. Bioorganic & Medicinal Chemistry, 2004, 12: 459~467.
[7] 谷记平, 刘仲华, 黄建安, 等. 单双液相下不同多酚氧化酶酶源对酶性合成茶黄素的研究[J]. 茶叶科学, 2007, 27(1): 76~82.
[8] 吴红梅. 多酚氧化酶酶源筛选及酶法制取茶色素研究[D]. 安徽农业大学2004届硕士论文, 安徽合肥, 2004.
[9] 李适. 微生物多酚氧化酶酶源筛选及其在茶黄素合成中的应用[Ｄ]. 湖南农业大学2006届硕士论文, 湖南长沙, 2006.
[10] 王坤波, 刘仲华, 黄建安. 儿茶素体外氧化制备茶黄素的研究[J]. 茶叶科学, 2004, 24(1): 53~59.
[11] Robertson Alastair. Effects of catechin concentration on the formation of black tea polyphenols during in vitro oxidation[J]. Phytochemistry, 1983, 22(4): 897~903.
[12] Robertson Alastair. Effects of physical and chemical conditions on the in vitro oxidation of tea leaf catechins[J]. Phytochemistry, 1983, 22(4): 889~896.
[13] Coggon Philip, Moss Gerald A, Sanderson Gary W. Tea catechol oxidase: isolation, purification and kinetic characterization [J]. Phytochemstry, 1973, 12: 1947~1955.

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Effects of Tea Catechin Proportions & Physico-chemical Conditions on the Enzymatic Synthesis of Theaflavin

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