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Journal of Tea Science ›› 2021, Vol. 41 ›› Issue (1): 101-112.doi: 10.13305/j.cnki.jts.2021.01.009

• Research Paper • Previous Articles     Next Articles

Screening, Identification and Culture Conditions of a High-virulent Isolate of Beauveria against Myllocerinus Aurolineatus

WANG Dingfeng, LI Liangde, LI Huiling, ZHANG Hui, WU Guangyuan*   

  1. Tea Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China
  • Received:2020-07-21 Revised:2020-11-01 Online:2021-02-15 Published:2021-02-23

Abstract: Myllocerinus aurolineatus (Coleoptera: Curculionidae) is one of the most important pests in tea plantation, and chemical control is the usual treatment method for this weevil at present. In order to explore alternative strategy for sustainable control of the weevil, dip method was employed to test the virulence of 12 isolates of Beauveria sp., which isolated from insects of Coleoptera. When inoculated with spore suspension (5.0×107 spores·mL-1), the strain Bbr1552 showed the highest virulence against the adults of M. aurolineatus, which caused the highest corrected mortality rates of 100%, shortest LT50 of 4.49 d and highest cadaver rates of 69.44%. The LC50 value was 1.55×106 spores·mL-1 on 7 d (25℃) after inoculation with Bbr1552. According to morphological identification and analysis of rDNA-ITS and Bloc sequences, the strain Bbr1552 was identified to be a B. brongniartii. Besides, the PPDA medium and 25℃ were the most optimal conditions for the colony growth and sporulation of the strain Bbr1552.

Key words: Myllocerinus aurolineatus, Beauveria brongniartii, virulence, identification, cultural condition

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