Rapid Determination of 24 Pesticide Residues in Tea by in-Syringe Dispersive Solid Phase Extraction-Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

MA Jiali; WANG Chen; CHEN Hongping; CHAI Yunfeng; ZHU Li; LIU Xin

doi:10.13305/j.cnki.jts.20210804.001

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Journal of Tea Science ›› 2021, Vol. 41 ›› Issue (5) : 717-730. DOI: 10.13305/j.cnki.jts.20210804.001

Rapid Determination of 24 Pesticide Residues in Tea by in-Syringe Dispersive Solid Phase Extraction-Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry

MA Jiali^1,2,3,4, WANG Chen^1,3,4,*, CHEN Hongping^1,3,4, CHAI Yunfeng^1,3,4, ZHU Li^1,3,4, LIU Xin^1,3,4,*

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Abstract

Based on the characteristics of tea matrix, a rapid pretreatment technology of in-syringe dispersive solid phase extraction (dSPE) was developed, and an analytical method based on ultra high performance liquid chromatography-tandem mass spectrometry was established for the detection of 24 pesticide residues in tea. Tea samples were extracted using acetonitrile, salted-out with MgSO₄, purified with primary secondary amine and graphitized carbon black as dispersive adsorbents in designed syringe devices, and detected by UPLC-MS/MS. Recoveries of 24 pesticides in black and green teas were 61.7%-98.8% at three spiked levels (0.01βmg·kg^-1, 0.05βmg·kg^-1 and 0.5βmg·kg^-1), which indicated high accuracy with the relative standard deviations of 0.4%-5.5%. The calibration curves of 24 pesticides in black and green teas showed good linearity with correlation coefficients (R²) higher than 0.995. The limits of detection (LOD) and the limits of quantitation (LOQ) were 0.05-5.36βμg·kg^-1 and 0.18-17.86βμg·kg^-1, respectively, which show high sensitivity of this method. Being simple, fast, less instrument required and time-saving, this method is suitable for quantification of multiple pesticide residues in tea.

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in-syringe dispersive solid phase extraction / pesticide residues / tea / ultra high performance liquid chromatography-tandem mass spectrometry

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MA Jiali, WANG Chen, CHEN Hongping, CHAI Yunfeng, ZHU Li, LIU Xin. Rapid Determination of 24 Pesticide Residues in Tea by in-Syringe Dispersive Solid Phase Extraction-Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry[J]. Journal of Tea Science. 2021, 41(5): 717-730 https://doi.org/10.13305/j.cnki.jts.20210804.001

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