The Expression Profiles of Chemosensory Protein 8 Orthologs in Two Closely Related Tea Geometrid Species, Ectropis obliqua Prout and Ectropis grisescens Warren

YAN Yuting; LI Yujie; WANG Qian; TANG Meijun; GUO Huawei; LI Hongliang; SUN Liang

doi:10.13305/j.cnki.jts.2022.02.007

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Journal of Tea Science ›› 2022, Vol. 42 ›› Issue (2) : 200-210. DOI: 10.13305/j.cnki.jts.2022.02.007

The Expression Profiles of Chemosensory Protein 8 Orthologs in Two Closely Related Tea Geometrid Species, Ectropis obliqua Prout and Ectropis grisescens Warren

YAN Yuting^1,2, LI Yujie^2,3, WANG Qian⁴, TANG Meijun², GUO Huawei², LI Hongliang^1,*, SUN Liang^2,*

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Abstract

Chemosensory proteins (CSPs) play crucial roles in insect chemosensory and non-chemosensory processes. Ectropis obliqua Prout and its sibling species, Ectropis grisescens Warren are serious lepidopteran moth pests in tea gardens and they cause destructive damages to tea plants. The development of greener and environmentally friendly pest managements would benefit from orthologous CSPs’ investigation on the interaction between two Ectropis species and tea plants. This study mainly examined the expression profiles of EgriCSP8, the orthologs of EoblCSP8 in E. grisescens by using the real-time quantitative PCR experiment. The qRT-PCR results show that EgriCSP8 and EoblCSP8 had a conserved larvae-enriched expression pattern. EgriCSP8 was mainly expressed in the heads of the third instar of E. grisescens larvae. Next, the expression levels of CSP8 were compared between E. obliqua and E. grisescens. The results show that the expression level of EoblCSP8 in E. obliqua was higher than that of EgriCSP8 in E. grisescens. In addition, the effects of Wolbachia and tea plant volatiles on CSP8 expression levels in E. obliqua and E. grisescens were also estimated. The results show that the EgriCSP8 expression level declined significantly when Wolbachia was removed in E. grisescens, which provided the first evidence that the insect CSPs’ expression is associated with Wolbachia. These findings laid a foundation for the future studies on the molecular mechanisms of chemosensory and coevolution between the geometrid sibling species and tea plants.

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Ectropis grisescens Warren / Ectropis obliqua Prout / Wolbachia / chemosensory protein / expression analysis

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YAN Yuting, LI Yujie, WANG Qian, TANG Meijun, GUO Huawei, LI Hongliang, SUN Liang. The Expression Profiles of Chemosensory Protein 8 Orthologs in Two Closely Related Tea Geometrid Species, Ectropis obliqua Prout and Ectropis grisescens Warren[J]. Journal of Tea Science. 2022, 42(2): 200-210 https://doi.org/10.13305/j.cnki.jts.2022.02.007

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