Phylogenetic Analysis of NUDX1 Gene Involved in Geraniol Biosynthesis

ZHOU Hanchen; YANG Jihong; XU Yujie; WU Qiong; LEI Pandeng

doi:10.13305/j.cnki.jts.2022.05.001

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Journal of Tea Science ›› 2022, Vol. 42 ›› Issue (5) : 638-648. DOI: 10.13305/j.cnki.jts.2022.05.001

Research Paper

Phylogenetic Analysis of NUDX1 Gene Involved in Geraniol Biosynthesis

ZHOU Hanchen, YANG Jihong, XU Yujie, WU Qiong, LEI Pandeng^*

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Abstract

Geraniol is an acyclic monoterpene alcohol, which plays key roles in plant-environment interactions, such as pest repelling, antimicrobial activity, and pollinator attraction as well as the aroma traits for tea plants. It was reported that a cytosolic Nudix hydrolase (NUDX1) catalyzes geranyl pyrophosphate (GPP) into geranyl monophosphate (GP), followed by dephosphorylation with an endogenous phosphatase to produce geraniol. Two homologues of AtNUDX1 were found in tea genome (CsNUDX1-cyto and CsNUDX1-chlo) with different subcellular location. Searching the homologues of AtNUDX1 on Phytozome shows that fifty-eight plant species contain the homologues of AtNUDX1 (with identities>64%). However, no homologue of AtNUDX1 was found in the genomes of grass species, with the exception of Brachypodium distachyon. We thus detected AR2000-enzymed geraniol in fresh leaves of rice, wheat, maize, and tea plants. The results show that free geraniol was undetectable in fresh leaves of rice, wheat and maize, where as young shoots of four tea cultivars had high levels of geraniol (0.87-4.12 μg·g^-1). Two CsNUDX1 genes were highly expressed in young tea leaves and had a positive correlation (above 0.7) with the accumulation of geraniol. This study shows that NUDX1s are widely present in plant genome, which are closely related to the formation of geraniol.

Key words

Camellia sinensis / NUDX1 gene / geraniol / phylogenetic analysis / Phytozome

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ZHOU Hanchen, YANG Jihong, XU Yujie, WU Qiong, LEI Pandeng. Phylogenetic Analysis of NUDX1 Gene Involved in Geraniol Biosynthesis[J]. Journal of Tea Science. 2022, 42(5): 638-648 https://doi.org/10.13305/j.cnki.jts.2022.05.001

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