Effects of Key Genes of Jasmonic Acid Synthesis and Transduction Pathway in Tea Plant on Terpenoids during Oolong Tea Processing

LU Li; ZHAN Dongmei; ZHOU Chengzhe; ZHU Chen; XIE Siyi; XU Kai; TIAN Caiyun; LAI Zhongxiong; GUO Yuqiong

doi:10.13305/j.cnki.jts.2023.01.007

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Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (1) : 91-108. DOI: 10.13305/j.cnki.jts.2023.01.007

Effects of Key Genes of Jasmonic Acid Synthesis and Transduction Pathway in Tea Plant on Terpenoids during Oolong Tea Processing

LU Li^1,2, ZHAN Dongmei^1,2, ZHOU Chengzhe^1,2,3, ZHU Chen^1,2,3, XIE Siyi^1,2, XU Kai^1,2, TIAN Caiyun^1,2, LAI Zhongxiong^1,3, GUO Yuqiong^1,2,*

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Abstract

In this study, we analyzed the key genes of jasmonic acid synthesis and signal transduction pathway in tea plants, explored their expression patterns and investigated their effects on the formation of terpenoids during oolong tea processing. There are 11 key gene families in this pathway, including 133 candidate genes. The analysis of cis-acting elements shows that the promoter regions of the key genes of the pathway contained a large number of cis-acting elements, including jasmonic acid response, damage response and anaerobic induction response. qRT-PCR analysis shows that most of the genes of this pathway showed an upward trend during the withering process, reached the highest in the second shaking process, significantly decreased in the fourth shaking process, and slightly increased in the process before green removal, and the key genes of this pathway could respond to various stresses during oolong tea processing. HS-SPME-GC-MS detected 73 terpenoids, mainly including linalool, geraniol and α-farnesene and other substances with flower and fruit flavor. Correlation analysis shows that CsLOX11, CsLOX12, CsAOS2, CsAOC1, CsACX4, CsACX8, CsMYC2-4, CsMYC2_15, CsMYC2_21 and β-pinene, limonene and myrcene are positively correlated, and CsOPR2, CsTPL6 and CsLUG4 are positively correlated with trans nerolidol, α-farnesene and ionone, among which CsTPL6 was significantly positively correlated with 35 terpenoids. In summary, the key genes of jasmonic acid synthesis and signal transduction pathway of tea plants are involved in regulating the formation of terpenoids during oolong tea processing, and this study laid a foundation for exploring the molecular mechanism of aroma formation during oolong tea processing.

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jasmonate synthesis and signal transduction / rocking green / tea plant / terpenoids / withering

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LU Li, ZHAN Dongmei, ZHOU Chengzhe, ZHU Chen, XIE Siyi, XU Kai, TIAN Caiyun, LAI Zhongxiong, GUO Yuqiong. Effects of Key Genes of Jasmonic Acid Synthesis and Transduction Pathway in Tea Plant on Terpenoids during Oolong Tea Processing[J]. Journal of Tea Science. 2023, 43(1): 91-108 https://doi.org/10.13305/j.cnki.jts.2023.01.007

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