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Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (2): 263-274.doi: 10.13305/j.cnki.jts.2023.02.001

• Research Paper • Previous Articles     Next Articles

Simultaneous Determination of Glyphosate, Glufosinate and Aminomethyl Phosphonic Acid Residues in Tea by Modified QuEChERS Method Coupled with UPLC-MS/MS

LI Ziqiang1, YANG Mei2, ZHANG Xinzhong2, LUO Fengjian2, LOU Zhengyun2, LIANG Shuang1,*   

  1. 1. College of Plant Protection, Jilin Agricultural University, Changchun 130118, China;
    2. Research Center of Quality Safety for Agricultural Products, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, China
  • Received:2023-01-13 Revised:2023-03-10 Online:2023-04-15 Published:2023-05-05

Abstract: An analytical method was developed for the simultaneous determination of glyphosate, glufosinate and aminomethyl phosphonic acid residues in black and green tea by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) coupled with a modified QuEChERS method. The samples were extracted twice with pure water, and the extraction solutions were purified by dispersive solid phase extraction with MWCNT, C18, PVPP and CNT-OH adsorbents. The mass spectrometry was performed in multiple reaction monitoring (MRM) mode and quantified by external standard method. The results show that glyphosate, glufosinate and aminomethyl phosphonic acid had a good linear relationship within a certain concentration range with correlation coefficients (R2) ranging from 0.999 3 to 1.000 0 in black and green tea. The limits of detection ( LOD ) of the method were 0.005 0, 0.030 mg·kg-1 and 0.030 mg·kg-1, respectively. The limits of quantitation (LOQ) were 0.050, 0.10 mg·kg-1 and 0.10 mg·kg-1, respectively. The average spiked recovery rates and the relative standard deviations (RSD) of pesticides were in the range of 81.6%-120.0% and 0.6%-13.6%, respectively. The method is simple, inexpensive, reproducible, sensitive and suitable for the determination of glyphosate, glufosinate and aminomethyl phosphonic acid in black and green tea.

Key words: QuEChERS, tea, glyphosate, glufosinate, aminomethyl phosphonic acid

CLC Number: