Identification of Transcription Factors Interacting with CsNCED2 Promoter and Their Response to Abiotic Stress

LI Jiasi; LIU Yingqing; ZHANG Yongheng; ZHANG Ying'ao; XIAO Yezi; LIU Lu; YU Youben

doi:10.13305/j.cnki.jts.2023.03.007

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Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (3) : 325-334. DOI: 10.13305/j.cnki.jts.2023.03.007

Research Paper

Identification of Transcription Factors Interacting with CsNCED2 Promoter and Their Response to Abiotic Stress

LI Jiasi, LIU Yingqing, ZHANG Yongheng, ZHANG Ying'ao, XIAO Yezi, LIU Lu, YU Youben^*

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Abstract

Nine cis epoxide carotenoid dioxygenase (NCED) is a key rate-limiting enzyme in abscisic acid (ABA) synthesis and widely involved in plant growth and development as well as abiotic stress response. CsNCED2 is involved in the response to drought and salt stress in tea plants, while the transcriptional regulation mechanism involved is still unclear. In this study, two transcription factors, CsDof5.4 and CsERF38, which binded to the CsNCED2 promoter were identified by yeast single hybrid (Y1H) library screening. Subcellular localization, yeast self-activation and luciferase (LUC) assay show that they were located in the cell nucleus and could activate the expression of CsNCED2. RT-qPCR results show that the expressions of CsERF38 and CsDof5.4 were highly correlated with CsNCED2 under salt stress. While under drought stress, only the expression of CsERF38was highly correlated with that of CsNCED2. In this study, two transcription factors (CsDof5.4 and CsERF38) binding to the CsNCED2 promoter were identified. Both drought and salt stresses could induce the expression of CsNCED2, thus participate in the abiotic stress response in tea plants.

Key words

CsNCED2 / expression analysis / tea plant / transcriptional regulation

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LI Jiasi, LIU Yingqing, ZHANG Yongheng, ZHANG Ying'ao, XIAO Yezi, LIU Lu, YU Youben. Identification of Transcription Factors Interacting with CsNCED2 Promoter and Their Response to Abiotic Stress[J]. Journal of Tea Science. 2023, 43(3): 325-334 https://doi.org/10.13305/j.cnki.jts.2023.03.007

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