Population Structure and Genetic Differences of Tea Germplasm Resources in Fujian

YANG Jun; ZHANG Lilan; ZHANG Wenjing; CHEN Linhai; ZHENG Guohua; LI Yijing; WANG Rangjian

doi:10.13305/j.cnki.jts.2023.06.006

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Journal of Tea Science ›› 2023, Vol. 43 ›› Issue (6) : 769-783. DOI: 10.13305/j.cnki.jts.2023.06.006

Research Paper

Population Structure and Genetic Differences of Tea Germplasm Resources in Fujian

YANG Jun¹, ZHANG Lilan¹, ZHANG Wenjing², CHEN Linhai³, ZHENG Guohua⁴, LI Yijing⁵, WANG Rangjian^1,*

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Abstract

The population genetic structure, genetic diversity, genetic differentiation, gene flow, molecular variance of 208 tea germplasm resources in Fujian were studied by using 38 pairs of SSR fluorescent primers, and the leaf traits were also investigated. The results show that the Nei’s genetic diversity index and Shannon’s information index of 208 tea resources in Fujian were 0.674 and 1.444, respectively. The average values of leaf area and length-width ratio were 27.442 cm² and 2.516, respectively. The genetic variation of tea germplasm in Fujian were mainly originated from individual genetic variation. The test materials were divided into 8 groups by the structure software analysis. The material sources in groups a, b, f, and h were single, while the material sources in groups c, d, e, and g were complex. The genetic background of tea population in different regions was similar. There were a total of 40 tea cultivars from Fujian in groups a, b, and e. Group a was mainly cultivars suitable for processing green tea. Group b was mainly cultivars suitable for processing oolong tea. The representative cultivars within group e were suitable for processing green tea. There is a certain correlation between the group attributes of group a, group b, group e and the suitable tea cultivars. Group c included the resources from Nanjing county, Yunxiao county and Pinghe county, with relatively close geographical locations. The group attributes are related to geographical sources. The gene flow value between group g and group e was 6.321, indicating frequent gene exchange between groups. The clustering of group similarity coefficients shows that group d and group b were closely related. The genetic differentiation between group h and other groups was obvious, and the characters of leaf area and leaf serration number were significantly different (P<0.05). The genetic relationship between group f and other groups was relatively distant, and there were significant differences in the characteristics of leaf serration number and leaf vein logarithm (P<0.05), showing that both group h and group f had certain uniqueness and need further identification. These research results provided a certain reference for the identification, screening, and utilization of tea germplasm resources in Fujian.

Key words

Fujian / genetic differentiation / population structure / tea plant

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YANG Jun, ZHANG Lilan, ZHANG Wenjing, CHEN Linhai, ZHENG Guohua, LI Yijing, WANG Rangjian. Population Structure and Genetic Differences of Tea Germplasm Resources in Fujian[J]. Journal of Tea Science. 2023, 43(6): 769-783 https://doi.org/10.13305/j.cnki.jts.2023.06.006

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