Identification of the Pathogen Causing New Twig Wilting on Tea Plants and Screening of Control Chemicals

WANG Juan; TU Yiyi; L&#x000dc; Wuyun; CHEN Yijia; LI Shipu; WANG Yuchun; CHEN Yanan

doi:10.13305/j.cnki.jts.2024.05.007

PDF(3342 KB)

Journal of Tea Science ›› 2024, Vol. 44 ›› Issue (5) : 807-815. DOI: 10.13305/j.cnki.jts.2024.05.007

Research Paper

Identification of the Pathogen Causing New Twig Wilting on Tea Plants and Screening of Control Chemicals

WANG Juan¹, TU Yiyi¹, LÜ Wuyun¹, CHEN Yijia¹, LI Shipu², WANG Yuchun^1,*, CHEN Yanan^1,*

Author information +

History +

Abstract

During summer and autumn, serious new twig wilting occurs on a large scale in ‘Jiaming No. 1' tea gardens of Ruian City, Wenzhou, Zhejiang Province, China. The disease initially appears as irregular brown spots on the twigs, which later spread to shoots and leaves, causing the tea shoots to wilt and the leaves to curl and shrink, and eventually the whole branch to die. In this study, a strain was isolated and purified using the tissue isolation method. Combining the morphological characteristics with the phylogenetic analyses based on the sequences of the internal transcribed spacer regions (ITS), β-tubulin (TUB2), and the translation elongation factor1 alpha (TEF1-α), the isolated strain was identified as Botryosphaeria fusispora. Laboratory pathogenicity tests show that B. fusispora was the pathogen causing the twig wilting disease present on ‘Jiaming No. 1'. These results indicate that B. fusispora can be a new record species causing twig wilting on tea plants. In addition, mycelial growth inhibition tests were conducted to examine the sensitivity of the pathogen to three commercial fungicides, including chlorothalonil, thiophanate-methyl and pyraclostrobin. Among the tested fungicides, thiophanate-methyl was found to be the most effective in suppressing the radial growth of the strain, with an EC₅₀of 1.91 μg·mL^-1, followed by pyraclostrobin, with an EC₅₀of 2.25 μg·mL^-1.

Key words

Botryosphaeria spp. / indoor toxicity test / tea plants / twig wilting

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

WANG Juan, TU Yiyi, LÜ Wuyun, CHEN Yijia, LI Shipu, WANG Yuchun, CHEN Yanan. Identification of the Pathogen Causing New Twig Wilting on Tea Plants and Screening of Control Chemicals[J]. Journal of Tea Science. 2024, 44(5): 807-815 https://doi.org/10.13305/j.cnki.jts.2024.05.007

References

[1] 马建强, 姚明哲, 陈亮. 茶树种质资源研究进展[J]. 茶叶科学, 2015, 35(1): 11-16.
Ma J Q, Yao M Z, Chen L.Research progress on germ-plasms of tea plant (Camellia sinensis)[J]. Journal of Tea Science, 2015, 35(1): 11-16.
[2] 梅宇, 梁晓. 2023年我国茶叶产销及进出口形势分析[J]. 中国茶叶, 2024, 46(4): 18-26.
Mei Y, Liang X.Analysis of China's tea production, sales, import and export situation in 2023[J]. China Tea, 2024, 46(4): 18-26.
[3] 徐云, 梅红, 林莉, 等. 茶树病害诊断与防治专家系统研制[J]. 农业系统科学与综合研究, 2003(2): 93-96, 100.
Xu Y, Mei H, Lin L, et al.The study and exploitation of diagnosed and controlled tea disease's expert system[J]. System Sciences and Comprehensive Studies in Agriculture, 2003(2): 93-96, 100.
[4] 陈宗懋, 陈雪芬. 世界茶树病原名录(续)[J]. 茶叶科学, 1989, 29(1): 73-88.
Chen Z M, Chen X F.A world list of pathogens reported on tea plant[J]. Journal of Tea Science, 1989, 29(1): 73-88.
[5] 陈雪芬. 我国茶树病害的发生趋势与绿色防控[J]. 中国茶叶, 2022, 44(6): 7-14.
Chen X F.The occurrence trend and green control of tea diseases in China[J]. China Tea, 2022, 44(6): 7-14.
[6] Jiang H, Zhang M, Zhou Y, et al.First report of Muyocopron laterale causing a new leaf disease of Camellia sinensis in China[J]. Beverage Plant Research, 2022, 2(1): 1-4. doi:10. 48130/bpr-2022-0010.
[7] Chen Y J, Wan Y H, Zou L J, et al.First report of leaf spot disease caused by Epicoccum layuense on Camellia sinensis in Chongqing, China[J]. Plant Disease, 2020, 104(7): 2029. doi: 10.1094/PDIS-09-19-1906-PDN.
[8] Yang D, Yao J, Wang B, et al.First report of Botrytis cinerea causing gray mold on tea (Camellia sinensis) in China[J]. Plant Disease, 2024, 108(1): 203. doi: 10.1094/PDIS-01-23-0022-PDN.
[9] López-Mora.Etiology of branch dieback and shoot blight of English Walnut caused by Botryosphaeriaceae and Diaporthe species in Southern Spain[J]. Plant Disease, 2020, 104(2): 533-550.
[10] Theissen F, Sydow H.Vorentwürfe zu den Pseudosphaeriales[J]. Annales Mycologici, 1918, 16(1/2): 1-34.
[11] Schoch C L, Shoemaker R A, Seifert K A, et al.A multigene phylogeny of the Dothideomycetes using four nuclear loci[J]. Mycologia, 2006, 98(6): 1041-1052.
[12] Slippers B, Boissin E, Phillips A, et al.Phylogenetic lineages in the Botryosphaeriales: a systematic and evolutionary framework[J]. Studies in Mycology, 2013, 76(1): 31-49.
[13] Phillips A J, Alves A, Abdollahzadeh J, et al.The Botryosphaeriaceae: genera and species known from culture[J]. Studies in Mycology, 2013, 76(1): 51-167.
[14] Rathnayaka A R, Chethana K W T, Phillips A J, et al. First report of Botryosphaeriaceae species on Camellia sinensis from Taiwan with a global checklist of Botryosphaeriaceae species on this host[J]. Chiang Mai Journal of Science, 2021, 48(5): 1199-1223.
[15] Jayawardena R S, Li X H, Xu W, et al.First report of Botryosphaeria dothidea causing leaf necrosis of Camellia sinensis in Fujian Province, China[J]. Plant Disease, 2016, 100(4): 854-854.
[16] 张永乐, 刘会香, 许永玉, 等. 围小丛壳菌山茶专化型与葡萄座腔菌复合侵染引致茶褐枯病[J]. 茶叶科学, 2018, 38(1): 87-93.
Zhang Y L, Liu H X, Xu Y Y, et al.The tea brown blight disease caused by co-infection of Glomerella cingulata f. sp. camelliae and Botryosphaeria dothidea[J]. Journal of Tea Science, 2018, 38(1): 87-93.
[17] 李力, 张渤, 范俐, 等. 侵染茶树的葡萄座腔菌分离鉴定及其拮抗菌筛选[J]. 宁德师范学院学报(自然科学版), 2023, 35(1): 75-80.
Li L, Zhang B, Fan L, et al.Isolation, identification and antagonistic bacterium screening of a fungal pathogen Botryosphaeria dothidea isolate infecting tea plant leaves[J]. Journal of Ningde Normal University (Natural Science), 2023, 35(1): 75-80.
[18] Cai L, Hyde K D, Taylor P W J, et al. A polyphasic approach for studying Colletotrichum[J]. Fungal Diversity, 2009, 39(1): 183-204.
[19] 吕务云. 禾谷镰刀菌细胞自噬途径相关基因的功能分析 [D]. 杭州: 浙江大学, 2019.
Lü W Y.Functional analysis of autophagy-related genes in Fusarium graminearum [D]. Hangzhou: Zhejiang University, 2019.
[20] Yukako H, Yuho A, Atsuko S, et al.Taxonomical study of noteworthy species of Botryosphaeria in Japan[J]. Mycobiology, 2021, 49(2): 122-132.
[21] Katoh K, Standley D M.MAFFT multiple sequence alignment software version 7: improvements in performance and usability[J]. Molecular Biology and Evolution, 2013, 30(4): 772-780.
[22] Tamura K, Peterson D, Peterson N, et al.MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods[J]. Molecular Biology and Evolution, 2011, 28(10): 2731-2739.
[23] Minh B Q, Schmidt H A, Chernomor O, et al.Corrigendum to: IQ-TREE 2: new models and efficient methods for phylogenetic inference in the genomic era[J]. Molecular Biology and Evolution, 2020, 37(5): 1530-1534.
[24] Li G Q, Liu F F, Li J Q, et al.Botryosphaeriaceae from Eucalyptus plantations and adjacent plants in China[J]. Persoonia, 2018, 40(1): 63-95.
[25] Crous P W, Slippers B, Wingfield M J, et al.Phylogenetic lineages in the Botryosphaeriaceae[J]. Studies in Mycology, 2006, 55(1): 235-253.
[26] 凌金锋. 荔枝病果相关的四属菌物鉴定及分子系统发育分析 [D]. 广州: 华南农业大学, 2019.
Ling J F.Identification and phylogenetic analysis of four fungal genera isolates associated with diseased litchi fruits in China [D]. Guangzhou: South China Agricultural University, 2019.
[27] Li G Q, Slippers B, Wingfield M J, et al.Variation in Botryosphaeriaceae from Eucalyptus plantations in Yunnan Province in southwestern China across a climatic gradient[J]. IMA Fungus, 2020, 11(22): 1-49. doi: 10. 1186/s43008-020-00043-x.
[28] Liu J K, Phookamsak R, Doilom M, et al.Towards a natural classification of Botryosphaeriales[J]. Fungal Diversity, 2012, 57(1): 149-210.
[29] 刘平平, 莫权辉, 叶开玉, 等. 广西猕猴桃果实病害病原菌分离与鉴定[J]. 中国南方果树, 2023, 52(4): 92-99.
Liu P P, Mo Q H, Ye K Y, et al.lsolation and identification of fruit diseases pathogens of kiwifruit in Guangxi[J]. South China Fruits, 2023, 52(4): 92-99.
[30] 褚睿天, 豆志鹏, 贺伟, 等. 引起蓝莓茎溃疡病的葡萄座腔菌属Botryosphaeria二新种[J]. 菌物学报, 2021, 40(3): 473-486.
Chu R T, Dou Z P, He W, et al.Two novel species of Botryosphaerla causing stem canker of blueberries from China[J]. Mycosystema, 2021, 40(3): 473-486.
[31] 叶碧欢, 沈建军, 李海波, 等. 植物提取物对香榧病原真菌的抑菌活性研究[J]. 中国森林病虫, 2023, 42(1): 1-5.
Ye B H, Shen J J, Li H B, et al.Antifungal activity of plant extracts against pathogenic fungi of Torreya grandis ‘Merrillii'[J]. Forest Pest and Disease, 2023, 42(1): 1-5.
[32] 周英, 欧阳慧, 蒋军喜, 等. 10种杀菌剂对葡萄座腔菌的室内毒力测定[J]. 中国南方果树, 2016, 45(6): 124-125, 130.
Zhou Y, Ouyang H, Jiang J X, et al.Indoor toxicity determination of 10 fungicides to Botryosphaeria dothidea[J]. South China Fruit, 2016, 45(6): 124-125, 130.
[33] 胡冲, 吴道军. 杀菌剂对樱花枝枯病菌葡萄座腔菌的室内毒力测定[J]. 农业与技术, 2020, 40(23): 19-20.
Hu C, Wu D J.Indoor toxicity determination of fungicides on Botryosphaeria dothidea of Cerasus cladosporioides[J]. Agriculture and Technology, 2020, 40(23): 19-20.
[34] 李诚, 蒋军喜, 冷建华, 等. 6种杀菌剂对猕猴桃主要腐烂病菌的室内毒力测定[J]. 中国南方果树, 2012, 41(1): 27-29.
Li C, Jiang J X, Leng J H, et al.Indoor toxicity test of six fungicides to main pathogenic fungi causing fruit rot of kiwifruit[J]. South China Fruits, 2012, 41(1): 27-29.
[35] 赵江, 余知和, 李其利, 等. 芒果葡萄座腔菌对3种杀菌剂的敏感性测定[J]. 南方农业学报, 2021, 52(4): 984-994.
Zhao J, Yu Z H, Li Q L, et al.Susceptibility of Botryosphaeriaceous isolates from mango to three fungicides[J]. Journal of Southern Agriculture, 2021, 52(4): 984-994.