SCoT标记分析陕西茶树资源的遗传多样性

doi:10.13305/j.cnki.jts.2016.02.003

Abstract

Abstract: Genetic diversity of 50 tea germplasm materials in Shaanxi was investigated. Taking 6 representative Shaanxi tea germplasm materials as templates, 38 of 121 SCoT marker primers were screened out, which showed specific bands and highly polymorphism. The results showed that SCoT markers could reveal high genetic diversity among tea germplasm, and each primer can detect out 4 to 17 alleles. On average, 11 allelic variations could be detected by each SCoT marker. 414 DNA fragments were amplified via 38 pairs of SCoT primer, of which 400 showed polymorphism and the ratio (PPB) was 96.62%. In addition, genetic polymorphism information content of each SCoT locus was between 0.56 and 0.99, the average of which was 0.90 for the tested materials, and the genetic similarity coefficient was between 0.59 and 0.71. The research showed that SCoT marker could be used in the genetic diversity research of tea germplasm. Basing on SCoT marker, genetic diversity level of these Shaanxi tea germplasms were relatively high in the genetic diversity.

Key words: tea (Camellia sinensis), SCoT, diversity

CLC Number:

S571.1
Q52

CHEN Xi, ZHANG Yu, LI Jiao, XI Yanjun, ZHANG Yanqing. Genetic Diversity Analysis of Tea Germplasm in Shaanxi Province Based on SCoT Marker[J]. Journal of Tea Science, 2016, 36(2): 131-138.

References

[1] Bertrand C, David J, Mackill C Y, et al.Start codon targeted (SCoT) polymorphism:a simple, novel DNA marker technique for generating gene-targeted markers in plants[J], Plant Mol Biol Rep, 2009, 27: 86-93.
[2] 熊发前, 蒋菁, 钟瑞春, 等. 目标起始密码子多态性(SCoT)分子标记技术在花生属中的应用[J]. 作物学报, 2010, 36(12): 2055-2061.
[3] 韩国辉. 基于EST-SSR、Genomic-SSR和SCoT标记的柑橘连锁图谱构建及杂种和多倍体遗传分析[D]. 重庆: 西南大学, 2012.
[4] 罗聪. 芒果SCoT分子标记与逆境和重要开花时间相关基因研究[D]. 南宁: 广西大学, 2012.
[5] 陈明辉, 张保青, 宋修鹏, 等. 宿根矮化病菌诱导甘蔗差异表达基因的cDNA-SCoT分析[J]. 作物学报, 2013, 39(6): 1119-1126.
[6] 蔡元保, 杨祥燕, 陈豪军, 等. SRAP 结合SCoT 标记分析番木瓜种质的遗传多样性[J]. 植物遗传资源学报, 2014, 15(2): 292-298.
[7] 侯小改, 王娟, 贾甜, 等. 牡丹SCoT分子标记正交优化及引物筛选[J]. 华北农学报, 2011, 26(5): 92-96.
[8] Chen L, Yamaguchi S.RAPD markers for discriminating tea germplasms at the inter-specific level in China[J]. Plant Breeding, 2005, 124: 404-409.
[9] 王雪萍, 马炳田, 齐桂年, 等. 四川主栽茶品种亲缘关系的RAPD分析[J]. 园艺学报, 2007, 34(1): 142-244.
[10] 沈程文, 宁正祥, 黄建安. 基于表型参数及SRAP标记的广东茶树种质遗传多样性[J]. 应用生态学报, 2009, 20(7): 1551-1558.
[11] 姚明哲, 陈亮, 马春雷, 等. ISSR和EST-SSR标记在检测中国、日本和肯尼亚茶树品种遗传多样性上的比较分析[J]. 分子植物育种, 2009, 7(5): 897-903.
[12] 沈程文, 黄建安, 赵世浩, 等. 利用SRAP和ISSR标记分析广东茶树种质资源的遗传多样性[J]. 核农学报, 2010, 24(5): 948-955.
[13] 乔婷婷, 马春雷, 周炎花, 等. 浙江省茶树地方品种与选分析[J]. 作物学报, 2010, 36(5): 744-753.
[14] 王丽鸳, 姜燕华, 段云裳, 等. 利用SSR分子标记分析茶树地方品种的遗传多样性[J]. 作物学报, 2010, 36(12): 2191-2195.
[15] 姚明哲, 乔婷婷, 马春雷, 等. EST-SSR标记与茶树表型性状关联的初步分析[J]. 茶叶科学, 2010, 30(1): 45-51.
[16] 夏丽飞, 陈林波, 梁名志, 等. 特异茶树资源生物碱测定及相关基因表达分析[J]. 西南农业学报, 2013, 26(3): 947-949.
[17] 刘本英, 孙雪梅, 李友勇, 等. 利用ISSR和EST-SSR标记分析茶树遗传多样性的比较[J]. 热带作物学报, 2009, 30(11): 1577-1581.
[18] 席春奕, 唐茜, 吴永胜, 等. 30份四川茶树种质资源遗传多样性与亲缘关系的SRAP分析[J]. 贵州农业科学, 2013, 41(2): 6-9.
[19] 晏嫦妤, 李家贤, 黄华林, 等. 几种DNA分子标记技术及其在茶树种质资源研究中的应用[J]. 生物技术通报, 2013(1): 63-66.
[20] Smith J S C, Chin E C L, Shu H.et al. An evaluation of the utility of SSR loci as molecular marker in maize (Zea mays L.): comparisons with data from RFLPs and pedigree[J]. Theor Appl Genet, 1997, 95: 163-173.
[21] Botstein D, White R L, Skolnick M, et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J]. Am J Hum Genet, 1980, 32: 314-331.

Genetic Diversity Analysis of Tea Germplasm in Shaanxi Province Based on SCoT Marker

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