Tea Polysaccharide Increased Doxorubicin Inhibition of Lung Cancer A549 Cells

WEI Nan; ZHU Qiangqiang; CHEN Jiming; LI Tong; LI Yifan; HUANG Yewei; WANG Xuanjun; SHENG Jun

doi:10.13305/j.cnki.jts.2016.05.005

PDF(918 KB)

Journal of Tea Science ›› 2016, Vol. 36 ›› Issue (5) : 477-483. DOI: 10.13305/j.cnki.jts.2016.05.005

Tea Polysaccharide Increased Doxorubicin Inhibition of Lung Cancer A549 Cells

WEI Nan^1,2, ZHU Qiangqiang^1,2,3, CHEN Jiming^1,2,3, LI Tong^1,2,3, LI Yifan^1,2, HUANG Yewei^1,2,3, MA Xiao^1,2,*, WANG Xuanjun^1,2, SHENG Jun^1,2

Author information +

History +

Abstract

Lung cancer has high incidence and mortality rates around the world and chemotherapy is widely applied in cancer treatment. Doxorubicin (DOX) is a kind of chemotherapy drug which commonly used in clinical trials. However, the drug has many side effects and may cause a dose-dependent injury in the long term. These side effects include irreversible cardiopulmonary, bone marrow suppression and so on. Moreover, its mufti-drug resistance also restricts it in certain clinical application. The effect of polysaccharides of tea extract combined with doxorubicin on human lung cancer cell line A549 were tested in this study. A549 was first cultured in vitro with different concentrations of DOX. After 24h culture, apotheosis ratio was determined by MTT. The results show that the DOX dose for the best inhibiting effect was 3 mg·L^-1 according to MTT detection. Three doses of DOX were selected to combine tea polysaccharide and suppress A549 cell survival. The combination of 2 mg·L^-1 DOX and 6 mg·L^-1 tea polysaccharide had been demonstrated to have better suppressing efficiency than 3 mg·L^-1 DOX alone. DOX could

Key words

antitumor / doxorubicin / tea polysaccharides

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

WEI Nan, ZHU Qiangqiang, CHEN Jiming, LI Tong, LI Yifan, HUANG Yewei, WANG Xuanjun, SHENG Jun. Tea Polysaccharide Increased Doxorubicin Inhibition of Lung Cancer A549 Cells[J]. Journal of Tea Science. 2016, 36(5): 477-483 https://doi.org/10.13305/j.cnki.jts.2016.05.005

References

[1] 许红, 王开贞, 王玉奎. 临床常用药物[M]. 济南: 山东科学技术出版社, 2005: 317.
[2] Bisht S, Maitra A.Dextran-doxorubicin chitosan nanoparticles for solid tumor therapy[J]. Wiley Interdiscip Rev Nanomed Nanobiotechnol, 2009, 1(4): 415-425.
[3] Batrakova EV, Li S, Brynskikh AM, et al.Effects of pluronic and doxorubicin on drug uptake, cellular metabolism, apoptosis and tumor inhibition in animal models of MDR cancers[J]. J Control Release, 2010, 143(3): 290-301.
[4] Feridooni T, Hotchkiss A, Remley-Carr S, et al, Cardiomyocyte specific ablation of p53 is not sufficient to block doxorubicin induced cardiac fibrosis and associated cytoskeletal change[J]. PLoS One, 2011, 6(7): e22801.
[5] Ajit Varki, Richard Cummings, Jeffrey Esko.Essentials of Glycobiology [M]. 北京: 清华大学出版社, 2002: 35-36.
[6] 周鹏, 谢明勇, 傅博强. 多糖的结构研究[J]. 南昌大学学报: 理科版, 2001, 25(2): 197-204.
[7] Jeremy M Berg, John L Tymoczko, Lubert Stryer, et al.Biochemistry[M]. W. H. Freeman & Co (sd), 2011: 2538.
[8] 严鸿德, 汪东风, 王泽农, 等. 茶叶深加工技术[M]. 北京: 中国轻工业出版社, 1998: 24-26.
[9] Richard Béliveau, Denis Gingras.Green tea: prevention and treatment of cancer by nutraceuticals[J]. The Lancet, 2004, 364: 1021-1022.
[10] Marcel W L, Koo Chi H.Pharmacological of green tea on the gastrointestinal system[J]. European Journal of Pharmacology, 2004, 500: 177-185.
[11] Laura Bravo.Polyphenols: chemistry, dietary sources, metabolism, and nutrition significance[J]. Nutr Rev, 1998, 56(11): 317-333.
[12] Wang SB, Yang CZ.Research advance in apoptosis induced by chemotherapy[J]. Chinese Journal of cancer, 2000, 19(12): 1173-1176.