Cloning and Functional Verification of Two MYB Transcription Factors in Tea Plant [Camellia sinensis (L.) ]

GONG Niandi; GUO Lili; WANG Hongxue; ZHAO Lei; WANG Jie; WANG Wenzhao; LIU Yajun; WANG Yunsheng; GAO Liping; XIA Tao

doi:10.13305/j.cnki.jts.2014.01.005

Journal of Tea Science >

2014 , Vol. 34 >Issue 1: 36 - 44

DOI: https://doi.org/10.13305/j.cnki.jts.2014.01.005

Cloning and Functional Verification of Two MYB Transcription Factors in Tea Plant [Camellia sinensis (L.) ]

GONG Niandi ,
GUO Lili ,
WANG Hongxue ,
ZHAO Lei ,
WANG Jie ,
WANG Wenzhao ,
LIU Yajun ,
WANG Yunsheng ,
GAO Liping ,
XIA Tao

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1. School of Biology Science, Anhui Agricultural University, Hefei 230036, China;
2. Key Lab of Tea Biochemistry and Biotechnology, Ministry of Education, Anhui Agricultural University, Hefei 230036, China

Received date: 2013-05-07

Revised date: 2013-06-26

Online published: 2019-09-03

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Abstract

R2R3-MYB in Sg4 subgroup probably participated in the regulation of lignin biosynthesis, which possibly play an important physiological role in plant growth and development. Two MYB transcription factors CsMYB4-5 and CsMYB4-6 in Sg4 subgroup were cloned by RACE technology. Investigation showed that the amino acid sequence of CsMYB4-5 showed 48.45% identity to AmMYB330 from Antirrhinum majus and 44.79% to AtMYB3 from Arabidopsis thaliana, while CsMYB4-6 showed 69.80% identity to AmMYB308 from Antirrhinum majus and 62.41% to AtMYB4 from Arabidopsis thaliana by bioinformatics analysis. The result of real-time fluorescent quantitative PCR showed that these two genes had all high expression levels in root while low expression levels in stem. The analysis of prokaryotic expression revealed that the recombinant CsMYB4-5 and CsMYB4-6 were expressed with a molecular weight of about 32 kD and 27 kD respectively. Compared with wild tobacco, transgenic tobacco with CsMYB4-6 gene appeared following symptoms: the veins of leaf tighten, lamina surface between veins with sags and crests, with white freckles in old leaf, but in transgenic tobacco with CsMYB4-5 gene the elder leaves turned yellow.

Key words： Camellia sinensis (L.); MYB transcription factor; expression analysis; prokaryotic expression analysis; transgenic tobacco analysis

Cite this article

GONG Niandi , GUO Lili , WANG Hongxue , ZHAO Lei , WANG Jie , WANG Wenzhao , LIU Yajun , WANG Yunsheng , GAO Liping , XIA Tao . Cloning and Functional Verification of Two MYB Transcription Factors in Tea Plant [Camellia sinensis (L.) ][J]. Journal of Tea Science, 2014 , 34(1) : 36 -44 . DOI: 10.13305/j.cnki.jts.2014.01.005

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