The aim of this study is to quickly and accurately identify different cultivars (or lines) of Fenghuang Dancong tea in which would construct a DNA fingerprint in different cultivars (or lines) of Fenghuang Dancong tea based on ISSR molecular marker. Firstly, 58 ISSR primers were screened with 4 cultivars, indicating 12 of which are polymorphic. After screening the optimal annealing temperature, 12 ISSR primers were used to amplify 39 Fenghuang Dancong tea resources. The results showed that by using 6 reliable ISSR primers, 84 DNA fragments, among which 78 DNA bands were polymorphic, of all 39 tea accessions were amplified. And the polymorphic proportion of DNA bands (PPB) was 92.86%, including the primer UBC843 with the richest polymorphic, and the average number of DNA bands amplified by each primer was 14. The average polymorphism information content (PIC) was 0.9456, and the genetic similarity coefficient of the 39 accessions ranged from 0.4063 to 0.9836. In order to identify all 39 accessions, it indicated that the combination efficiency of UBC827/UBC846 was the highest by making combination of the 6 core ISSR primers. By connecting the name of cultivars, the national area code, the sampling sites, the name of the highest efficient primer combination and the ISSR data code, the DNA fingerprint codes of 39 Fenghuang Dancong tea were built. And then, the relevant information of the sample such as its flavor type, altitude, ISSR-PCR amplified picture and so on, were all typed in the software to generate the two-dimensional code fingerprint. The construction of the DNA fingerprint in different cultivars (or lines) of Fenghuang Dancong tea could offer data support for the protection of the cultivars rights and interests.
MA Ruijun
,
MEI Hongjuan
,
ZHUANG Donghong
,
YANG Peikui
,
WU Qinghan
. Construction of DNA Fingerprints in Fenghuang Dancong Tea of Different Cultivars (or Lines)[J]. Journal of Tea Science, 2014
, 34(5)
: 515
-524
.
DOI: 10.13305/j.cnki.jts.2014.05.013
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