A method for determination of total organic acids in tea plant by automatic potentiometic titrator was established in this study. And the content of total organic acids in bud, young leaf, mature leaf and withered leaf were determined by this method. The results showed that after extracting by ethanol, concentrated under reduced pressure, the concentrated solution was alkalized and acidified, then detected with automatic potentiometic titrator, the samples were spiked at the level of 2.0-45.0 mg·g-1, and the recovery of organic acids range from 96.0%-99.6% with RSD less than 5.7%. This simple and sensitive method meets with the analytical requirement to total organic acids in tea. The content of total organic acids in bud, young leaf, mature leaf and withered leaf was 44.9, 20.1, 21.1 and 1.99 mg·g-1 respectively.
ZHU Xujun
,
YANG Pingping
,
XU Renkou
,
WANG Yuhua
,
LI Xinghui
. A Method for Determination of Total Organic Acids in Tea Plant with Automatic Potentiometic Titration[J]. Journal of Tea Science, 2014
, 34(6)
: 572
-576
.
DOI: 10.13305/j.cnki.jts.2014.06.018
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