The caffeine content of 60 individuals of a low-caffeine population of tea plant was analyzed using HPLC and the molecular variance was studied using 42 EST-SSR markers, respectively. The results showed that the caffeine content of fresh weight ranged from 0.38% to 1.08%. Five individuals had lower caffeine content than female parent, and they can be used as breeding materials for further screening low-caffeine tea cultivars. One hundred and twenty-nine alleles were detected, each pair of primers could detect 2 to 7 alleles, an average of 3.86. The average number of Shannon-Weaver index (I) was 0.65. The polymorphism information content (PIC) of EST-SSR markers varied from 0.03 to 0.68, with average of 0.33. Three SSR markers, TM089, TM200 and TM211, related to variation of caffeine content were preliminary identified. It would be of important significance for screening excellent genetic resources and breeding new low-caffeine tea cultivars.
WANG Xue-min
,
YAO Ming-zhe
,
JIN Ji-qiang
,
MA Chun-lei
,
CHEN Liang
. Analysis of Caffeine Content and Molecular Variance of Low-caffeine Tea Plants[J]. Journal of Tea Science, 2012
, 32(3)
: 276
-282
.
DOI: 10.13305/j.cnki.jts.2012.03.013
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