Expression Analysis of Auxin-related Genes at Different Winter Dormant Stages of Axillary Buds in Tea Plant (Camellia sinensis)

WANG Xin-chao; MA Chun-lei; YANG Ya-jun; CAO Hong-li; HAO Xin-yuan; JIN Ji-qiang

doi:10.13305/j.cnki.jts.2012.06.012

2012 , Vol. 32 >Issue 6: 509 - 516

DOI: https://doi.org/10.13305/j.cnki.jts.2012.06.012

Expression Analysis of Auxin-related Genes at Different Winter Dormant Stages of Axillary Buds in Tea Plant (Camellia sinensis)

WANG Xin-chao ,
MA Chun-lei ,
YANG Ya-jun ,
CAO Hong-li ,
HAO Xin-yuan ,
JIN Ji-qiang

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Tea Research Institute of Chinese Academy of Agricultural Sciences, National Center for Tea Improvement, Key Laboratory of Tea Plant Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China

Received date: 2012-05-30

Revised date: 2012-07-04

Online published: 2019-09-05

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Abstract

The expression patterns of some auxin-related genes, including auxin response factors (ARFs), primary auxin responsive genes, auxin-regulated genes, auxin influx carrier gene and auxin-binding protein (ABP) gene, were studied in different stages of tea plant axillary bud dormancy and its release. The results showed that (1) the expression levels of five in six ARF homologous EST fragments were up-regulated in the deep dormancy and down-regulated after sprouted, the level of the last one showed the opposite expression levels. (2) There were significant differences among the expression patterns of 3 ESTs which are homologous with primary auxin responsive genes. EST which is homologous with SAURs was up-regulated in the dormancy stage but down-regulated after dormancy release. On the contrary, the 2 ESTs which are homologous with GH3 and Aux/IAAs were up-regulated in sprouting stage while down-regulated in dormant stages and after sprouting stages. (3) The CsGLP1 gene which was homogenous with ABPs has a lower expression level in the dormancy stage but maintain rising trend after germination.(4) The EST which is homogenous with auxin influx carrier gene showed the highest expression levels during the deep dormant stage but maintain lower levels in other stages. (5) Two auxin-regulated ESTs showed different expression patterns. The expression levels of auxin-induced EST were higher in the dormant stages than those in the sprouting stages, while the levels of the other auxin-repressed EST were higher in the sprouting stage than that in the dormant stages. These results indicated that auxin promote the dormancy of tea axillary buds in winter.

Key words： auxin-related gene; bud dormancy and budbreak; expression analysis; tea plant (Camellia sinensis)

Cite this article

WANG Xin-chao , MA Chun-lei , YANG Ya-jun , CAO Hong-li , HAO Xin-yuan , JIN Ji-qiang . Expression Analysis of Auxin-related Genes at Different Winter Dormant Stages of Axillary Buds in Tea Plant (Camellia sinensis)[J]. Journal of Tea Science, 2012 , 32(6) : 509 -516 . DOI: 10.13305/j.cnki.jts.2012.06.012

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