A method to measure nicotinic acid by high performance liquid chromatography was investigated in this work. Method: using Agilent 1100 HPLC series, C18 column (4.6mm×150mm), mobile phase of MeOH∶0.05mol/L KH2PO4 (1∶9), with flow rate of 1.0mL/min, and the detection wavelength at 266nm. The nicotinic acid curve was linear with R2=0.9915. The average recovery rate for Nicotinic acid was 99.38% with RSD 2.18%(n=4). Conclusion: the method is simple and has good reproducibility. It coulde be used for analysis of nicotinic acid in tea leaves.
PEI Ting
. Determination of Nicotinic Acid in Tea Leaves by HPLC[J]. Journal of Tea Science, 2011
, 31(2)
: 124
-128
.
DOI: 10.13305/j.cnki.jts.2011.02.008
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