Tea Cultivar Dayelong is a vegetative propagation cultivar of large-leaf and budless mutated from a stock plant of Ningzhou population. The paraffin section of mature leaves and buds of different period from Dayelong and its stock plant was investigated. Results showed that the palisade cell was rather long, the layers of spongy cell increased. Dayelong do not have a flower bud is due to all the buds always maintain the situation of vegetative bud. Three actin gene fragments (CS-ACT1, CS-ACT2, CS-ACT3) of tea plant were cloned for the first time. They encoding 225 amino acids. Dayelong, its stock plant and other 500 Actin squences obtained by BlastX analysis showed four amino acids of Dayelong are different from those of corresponding sites on conservative domains F, G and H. The Dayelong and its stock plant for AFLP analysis showed that the band numbers of Dayelong is fewer than the stock plant and has its own specific band. EST-SSR molecular markers technique was used to analyze Dayelong and other cultivars, the results showed that four cultivars of Xiushui including the Dayelong are similar to Longjing 43 in genetic basis.
XU Ling-ling
,
ZHANG Mei-yun
,
LI Tong- jian
,
QIN Hong-xia
,
ZHAO Zhong-wei
,
FAN Qi-shui
,
LIAO Liang
,
ZHANG Da-ming
. Study on Anatomy, Actin Gene and Molecular Marker of Tea Cultivar Dayelong[J]. Journal of Tea Science, 2009
, 29(4)
: 263
-270
.
DOI: 10.13305/j.cnki.jts.2009.4.003
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