According to the relevant complete mRNA sequence of α-tubulin gene of Camellia sinensis (accession NO. DQ340766), the coding region of α-tubulin gene was amplified using RT-PCR method, and its products were expressed in Escherichia coli using the pET-32a(+) vector. The fusion protein was expressed in the form of inclusion body when induced with isopropyl-D-thiogalatopyranoside (IPTG), then was purified to immunize the rabbit to produce the polyclonal antibody against α-tubulin. The specificity of the antibody was confirmed by Western blot analysis. The results of Western blot revealed well specificity of the antibody against α-tubulin in Camellia sinensis plant.
TAN Zhen
,
TONG Xin
,
FANG Chao
,
JIANG Chang-jun
,
CHEN Cong
. Prokaryotic Expression of α-tubulin Gene of Camellia sinensis and Preparation of α-tubulin Polyclonal Antibody[J]. Journal of Tea Science, 2009
, 29(5)
: 336
-340
.
DOI: 10.13305/j.cnki.jts.2009.5.002
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