EGCG3″Me was isolated by preparative HPLC system from tea and identified by NMR and LC/MS. The influence of different extracting method on the EGCG3″Me content in tea was discussed, and a simple high performance liquid chromatography analytical method of EGCG3″Me was developed. Results showed that the analytical method showed a good linear relationship between the peak area and content of EGCG3″Me (R2=0.9998) and high reproducibility. The average recovery rate of EGCG3″Me was 95.48% and the relative standard deviation(RSD)was 3.31%. The EGCG3″Me concentration in tea infusion can reach highest when the tea sample was immersed in distilled water for 5 min at 90℃.
SUN Ye-liang
,
LV Hai-peng
,
LIN Zhi
,
LI Xing-hui
. Study on the Analytical Method of EGCG 3″Me by HPLC in Tea[J]. Journal of Tea Science, 2009
, 29(5)
: 379
-384
.
DOI: 10.13305/j.cnki.jts.2009.5.008
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