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Analysis of Genetic Relationships Among “Rucheng Baimao Cha” Plants with RAPD Method

  • LI Xing-hui ,
  • LIU Chun-lin ,
  • SHI Zhao-peng ,
  • LUO Jun-wu ,
  • SHEN Cheng-wen ,
  • GONG-zhihua ,
  • CHEN-xuan
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  • 1. Nanjing Agricultural University, Nangjing 210095, China;
    2. Hunan Agricultural University, Changsha 410128, China

Received date: 2004-02-18

  Revised date: 2004-03-15

  Online published: 2019-09-11

Abstract

The genetic relationships of the individual plants among the “Rucheng Baimao Cha” population were studied with the RAPD method. The results were as follows: The maximum number of PCR bands of the individual plants among the “Rucheng Baimao Cha” population was 13,and the minimum number was 10. The maximum DNA polymorphism amplified by RAPD-PCR was 92.31%, while the minimum was 70.00%; the average was 85.40%. This result showed the abundant genetic diversities. The maximum genetic distances among individual plants of the “Rucheng Baimao Cha” population was 0.5791; the minimum one was 0.0882. The result from the DNA molecular dendrogram showed that the variation among individual plants of“Rucheng Baimao Cha” population was continuous. The cluster analysis by UPGMA showed that the “Rucheng Baimao Cha”population could be divided into 6 groups. This result matched the morphologic characteristics of the population. From the DNA molecular dendrogram it was showed that the key germplasm groups were protected in the Rucheng Baimao Cha Demonstration Farm in Rucheng county, Hunan province, P. R. China.

Cite this article

LI Xing-hui , LIU Chun-lin , SHI Zhao-peng , LUO Jun-wu , SHEN Cheng-wen , GONG-zhihua , CHEN-xuan . Analysis of Genetic Relationships Among “Rucheng Baimao Cha” Plants with RAPD Method[J]. Journal of Tea Science, 2004 , 24(1) : 33 -36 . DOI: 10.13305/j.cnki.jts.2004.01.006

References

[1] 刘贵芳. 汝城大叶白毛茶野生资源的考察[J]. 中国茶叶,1983, (2): 14-15.
[2] 叶创兴. 山茶属三新种[J].中山大学学报(自然科学版),1987, (1): 17-20.
[3] 闵天禄.山茶属茶组植物的订正[J].云南植物研究,1992, (2):115-132.
[4] 张宏达. 茶叶植物资源的订正[J]. 中山大学学报(自然科学版), 1984, (1): 1-12.
[5] 钱韦, 葛颂, 洪德元. 采用RAPD和ISSR标记探讨中国疣粒野生稻的遗传多样性[J]. 植物学报, 2000, 42(7): 741-750.
[6] Nei M,Li W H. Mathematical models for studying genetic distance in terms of restrication endonucleases[J]. Proceeding of the National Academy of Sciences, 1979, 76: 5269-5273.
[7] 陈亮,杨亚军,虞富莲, 等. 15个茶树品种遗传多样性的RAPD分析[J]. 茶叶科学,1998, 18(1):21-27.
[8] Wachira, F.N. Characterisation and estimation of genetic relatedness among heterogenous population of commercial tea clones by random amplification of genomic DNA samples[J]. Tea, 1997(18): 11-20.
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