Welcome to Journal of Tea Science,Today is

Isolation and Identification of “Jinhua” Fungi from the Loose Tea with“Fungus Growing”

  • HUANG Hao ,
  • LIU Zhong-hua ,
  • HUANG Jian-an ,
  • LI Shi ,
  • LI Juan ,
  • WU Yang ,
  • DENG Ting-ting ,
  • GONG-Xue
Expand
  • National Research Center of Engineering & Technology for Utilization of Functional Ingredients from Botanicals, Key Lab of Tea Science of Ministry of Education of Hunan Agricultural University, Changsha 410128, China

Received date: 2010-04-22

  Revised date: 2010-06-17

  Online published: 2019-09-11

Abstract

A fungi called “Jinhua” that separated from Jing Xiangyi brick tea was produced by Hunan Yiyang Tea Factory in 2008. Inoculating the fungi to the raw material of Yiping Fuzhuan tea of 2008 with “Fungus growing” by controlling the conditions of environment. A strain of “Jinhua” fungi was isolated from the loose tea with fungus growing as a tested fungi through subculture purification. The epigamous and phorozoon of the tested fungi was observed with microscope, and the ascospores, and conidia of the tested fungi was observed SEM, and the tested fungi was identified in the molecular level by DNA sequencing. According to the characteristic of the morphology, microscopic, and SEM observed as well as the DNA sequencing of the tested strains, it was identified as Eurotium cristatum.

Cite this article

HUANG Hao , LIU Zhong-hua , HUANG Jian-an , LI Shi , LI Juan , WU Yang , DENG Ting-ting , GONG-Xue . Isolation and Identification of “Jinhua” Fungi from the Loose Tea with“Fungus Growing”[J]. Journal of Tea Science, 2010 , 30(5) : 350 -354 . DOI: 10.13305/j.cnki.jts.2010.05.004

References

[1] 肖文军, 傅冬和, 任国谱, 等. 茯茶毒理学试验报告[J]. 茶叶科学, 2007, 27(4): 307-310.
[2] 王志刚, 童哲, 程苏云. 茯砖茶中霉菌的产毒性研究I散囊菌培养液的毒性[J]. 茶叶科学, 1992, 12(1): 65-67.
[3] 傅冬和, 刘仲华, 黄建安, 等. 高通量筛选研究茯砖茶对FXR模型的作用[J]. 食品科学, 2007, 28(5): 331-334.
[4] 王志刚, 童哲, 程苏云. 茯砖茶中霉菌的产毒性研究Ⅱ冠突散囊菌的菌体毒性测定[J]. 茶叶科学, 1994, 14(1): 69.
[5] 齐祖同, 孙曾美. 茯砖茶中优势菌种的鉴定[J]. 真菌学报, 1990, 9(3): 176-179.
[6] 周传云. 食品微生物学实验技术[M]. 长沙: 湖南农业大学食品科技学院微生物教研室, 1999.
[7] 朱衡, 瞿峰. 利用氯化苄提取适于分子生物学分析的真菌DNA[J]. 真菌学报, 1994, 13(1): 34-40.
[8] White T, Bruns T, Lee S, et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[C]//Innis MA, Gelfand DH, Sninsky J, et al. PCR protocols: a guide to methods and applications. New York: Academic Press, 1990: 315-322.
[9] 郑雪松, 杨虹, 李道棠, 等. 基因间隔序列(ITS)在细菌分类鉴定和种群分析中的应用[J]. 应用与环境生物学报, 2003, 9(6): 678-684.
[10] 邢来君, 李明春. 普通真菌学[M]. 北京: 高等教育出版社, 1999, 271.
[11] 杨苏声, 周俊初. 微生物生物学[M]. 北京: 科学出版社, 2004.
[12] 齐祖同. 中国真菌志: 第五卷: 曲霉及相关有性型[M]. 北京: 科学出版社. 1997.
[13] Raper K, Fennell D, Austwick P.The Genus Aspergillus[M]. Baltimore: Williams & Wilkins Baltimore, 1965.
Outlines

/