The 18-week old roman layers were applied in this investigation. 1620 roman layers were randomly distributed to nine groups, each group includes three replications and each replication has sixty chickens. The total period lasted for 98 days. Control group was fed basal diet,the other eight groups received respectively diets with the addition of 2, 4, 6, 8 g/kg green tea powder (GTP) and 0.5, 1.0, 1.5, 2.0 g/kg tea polyphenols (TPs). The aim of this study was to investigate the effects of GTP and TPs on total cholesterol (TC) and total triglyceride (TG) levels of leg muscle, pectoral muscle, egg and blood of roman layers. To determine the samples on a regular basis, the results showed that the addition of 6 g/kg,8 g/kg GTP, 1.5 g/kg and 2.0 g/kg TPs into diets can reduce TC and TG level in organism, egg and blood, the largest trend of reduction occurred from eight to ten weeks. The effect of TPs was better than that of GTP. This research would provide the groundwork for the development of GTP applied in chicken feed.