Genetic diversity on 34 Fenghuang-Dancong tea plants were evaluated by AFLP fingerprinting using 5 primer combinations. The 5 primer combinations generated 438 bands and 87.6 bands were amplified by each primer pair on average, 348 bands(79.3%) polymorphic AFLP markers were detected, The result indicated that the genetic distance(GD) based on AFLP data by using DPS2000 was significant. The varience range of GD among the 34 Fenghuang-Dancong was 0.13~0.49. The GD(0.49) is highest between Zimaohuangzhixiang and Funanmilan and between Baiyedancong and Tongtianxiang, however, the GD(0.13) between Da-ansongcha and Zongsuojia is low. The dendrogram indicated that the genetic of 34 Fenghuang-Dancong germplasms have no certain relation with the aroma type.
XIAO Li-zheng
,
YAN Chang-yu
,
LI Jia-xian
,
LUO Jun-wu
,
HE Yu-mei
,
ZHAO Chao-yi
. AFLP Analysis on Genetic Diversity of Fenghuang-Dancong Tea Plant Germplasms[J]. Journal of Tea Science, 2007
, 27(4)
: 280
-285
.
DOI: 10.13305/j.cnki.jts.2007.04.002
[1] Barbier, E B, Burgess, J C, Folke, C.Paradise Lost? The Ecological Economics of Biodiversity[M]. London: Earthscan Publications Ltd, 1994.
[2] 鲁凤娟. 梨属植物AFLP反应体系的建立及其应用[D]. 河北农业大学硕士学位论文, 2004.
[3] 黄建安. 茶树分子遗传图谱构建及多酚氧化酶基因的SNP研究[D]. 湖南农业大学博士学位论文, 2004.
[4] 周李华. 广东茶树种质资源遗传多样性AFLP分析[D]. 湖南农业大学硕士学位论文, 2006.
[5] 黄建安, 黄意欢, 罗军武, 等. 茶树基因组DNA的高效提取方法[J]. 湖南农业大学学报, 2003, 29(5): 402~407.
[6] Massimo L, Fabrizio G, Serena I,et al. AFLP analysis as biomarker of exposure to organic and inorganic genotoxic substances in plant[J]. Chemosphere, 2003, 52(7): 1183~1188.
[7] 易克. 西瓜遗传图谱的构建及其主要农艺性状的基因定位[D]. 湖南农业大学博士学位论文, 2002.
[8] Nei M, Li W.Mathematical model for studying genetic variation in terms of restriction endonuclease[J]. Proc Natl Acad Sci USA, 1979, 76: 5269~5273.
[9] 唐启义, 冯明光. 实用统计分析及其DPS数据处理系统[M]. 北京:科学出版社, 2002.
[10] 陈守良, 徐克学. 应用数量分类探讨鹅冠草属的归属问题[J]. 植物分类学报, 1989, 27(3): 190~196.
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