Welcome to Journal of Tea Science,Today is

Study of Green Tea Polysaccharides on Lipid Peroxidation of Plasma Lipoproteins

  • YIN Xue-zhe ,
  • XU Hui-xian ,
  • JIN Hua
Expand
  • Affiliated Hospital of Yanbian University College of Medicine, Yanji 133000, China

Received date: 2007-05-18

  Revised date: 2007-08-16

  Online published: 2019-09-11

Abstract

The oxygen free radical scavenging activities and anti-lipoprotein oxidative effects of green tea polysaccharides were studied. ROO·, O2-· and OH· scavenging activities were studied with chemiluminescence analysis, H2O2 scavenging activity was determined by spectrophotometry. Very low density lipoprotein (VLDL), low density lipoprotein (LDL) and high density lipoprotein (HDL) were isolated from fasting plasma by sequential ultracentrifugation at 0βh and 2βh after 20 healthy volunteers ingested green tea polysaccharides and degrees of peroxidization and susceptibilities of lipoproteins to oxidation were investigated. The results showed that green tea polysaccharides exhibited scavenging effects on ROO·, O2-·, OH· and H2O2 in vitro, significantly decreased lipid peroxides of plasma, VLDL, LDL and HDL; noticeably prolonged lag phage of LDL oxidation curve, indicating reduced susceptibility of LDL to oxidation. It is suggested that green tea polysaccharides can increase antioxidant capacities of plasma and lipoproteins, therefore may show the function of preventing cardiovascular disorders and delaying ageing.

Cite this article

YIN Xue-zhe , XU Hui-xian , JIN Hua . Study of Green Tea Polysaccharides on Lipid Peroxidation of Plasma Lipoproteins[J]. Journal of Tea Science, 2007 , 27(4) : 299 -301 . DOI: 10.13305/j.cnki.jts.2007.04.004

References

[1] 陈海霞, 谢笔钧. 茶多糖对小鼠实验性糖尿病的防治作用[J]. 营养学报, 2002, 24(1): 85~86.
[2] Tadakazu T, Tomoki U, Hitoshi K, et al. The chemical properties and functional effects of polysaccharides dissolved in green tea infusion[J]. Nippon Shokuhin Kagaku Kaishi, 1998, 45(4): 270~272.
[3] 郑荣梁. 自由基生命科学进展, 第五集[M]. 北京: 原子能出版社, 1997.
[4] Murcia MA, Jimenez AM, Martinez-Tome M.Evaluation of the antioxidant properties of Mediterranean and tropical fruits compared with common food additives[J]. J Food Prot, 2001, 64(12): 2037~2046.
[5] Havel RJ, Eder HA, Bragdon JH.The distribution and chemical composition of ultracentrifugally separated lipoproteins in human serum[J]. J Clin Invest, 1955, 51: 1486~1494.
[6] Markwell MAK, Haas SM, Bieber LL, et al. A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples[J]. Anal Biochem, 1978, 87: 206~210.
[7] Esterbauer H, Striegl G, Puhl H, et al. Continuous monitoring of in vitro oxidation of human low-density lipoproteins[J]. Free Radical Res Commun, 1989, 6: 67~75.
Outlines

/