The hot water extract (crude extract) of kudingcha made from Ilex kudincha C. J. Tseng and its four fractions of chloroform, ethyl acetate, n-butanol, and water were prepared. The total polyphenol contents and the antioxidant activity of the extracts were determined by Folin-Ciocalteu method and DPPH assay, FRAP assay, and TEAC assay, respectively. The results showed that kudingcha extracts contained high polyphenol content and showed potent antioxidant activity. The antioxidant activities of crude extract and its fractions decreased in the order of ethyl acetate fraction>butanol fraction>crude extract>water fraction>chloroform fraction according to the DPPH assay and FRAP assay, which were the same with the exception of the rank order of water fraction and chloroform fraction obtained from TEAC assay. Satisfactory correlations between the total polyphenol content and antioxidant activity and between measuring methods of antioxidant activity were observed. It suggests that the polyphenols in the extracts are largely responsible for the antioxidant activities.
LIU Li-xiang
,
LIANG Xing-fei
,
SUN Yi
,
Tanguy Laura
,
YE Hong
,
ZENG Xiao-xiong
. Determination of Polyphenolic Content and Antioxidant Activity of Kudingcha Made from Ilex kudincha C. J. Tseng[J]. Journal of Tea Science, 2008
, 28(4)
: 289
-293
.
DOI: 10.13305/j.cnki.jts.2008.04.009
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