Germination of pollens and growth of pollen tubes were observed in vivo after self- or cross-pollination in the style of clonal tea cultivar Longjing43 using fluorescence microscopy by aniline blue staining. The results showed that pollens in self-pollination germinated normally, and it was the same as that in cross-pollination. There was no significant difference in growth characteristics of pollen tubes between self- and cross-pollination during 24 hours after pollinating,but thereafter the growth speed of pollen tubes in self-pollination was slower than that in cross-pollination and was finally stoped in the style base, and meanwhile there were some incompatibility-phenomenons that the form of pollen tube apex was abnormal and fluoresced more obviously. It was suggested that the inhibited morphological manifestations of pollen tubes in self-pollination accorded with the characteristics of floral gametophytic self-incompatibility.
WANG Yu
,
JIANG Chang-jun
,
ZHANG He-yu
. Observation on the Self-incompatibility of Pollen Tubes in Self-pollination of Tea Plant in Style in vivo[J]. Journal of Tea Science, 2008
, 28(6)
: 429
-435
.
DOI: 10.13305/j.cnki.jts.2008.06.008
[1] Matton DP, Nass N, Clarke AE, et al. Self-incompatibility: How plants avoid illegitimate offspring[J]. Proc Natl Acad Sci USA, 1994, 91: 1992~1997.
[2] 吴华清, 张绍铃, 李晓, 等. 植物自交不亲和性的分子生物学进展[J]. 南京农业大学学报, 2006, 29(4): 119~126.
[3] 江昌俊. 茶树育种学[M]. 北京: 中国农业出版社, 2005.
[4] 董丽娟. 茶树杂交育种研究进展[J]. 茶叶科学, 2001, 21(1): 7~10.
[5] 刘祖生, 梁月荣. 茶树受精作用的研究[J]. 茶叶科学, 1987, 7(2): 19~22.
[6] Kao T H, McCubbin A G. How flowering plants discriminate between self and non-self pollen to prevent inbreeding[J]. Proc Natl Acad Sci USA, 1996, 93: 12059~12065.
[7] 王春雷, 张绍铃. 荧光标记在植物花粉管构造及生长特性研究中的应用[J]. 西北植物学报, 2007, 27(2): 407~413.
[8] 胡适宜. 植物胚胎学实验方法(五)检查花粉在柱头上萌发和花粉管在花拄中生长的制片法[J]. 植物学通报, 1994, ll: 58~60.
[9] 郝怀庆, 王钦丽, 陈艳梅, 等. 花粉管细胞壁结构及胞质运动[J]. 植物学通报, 2003, 20(3): 270~279.
[10] 中国科学技术情报研究所重庆分所. 国外茶叶资料[M]. 重庆: 科学技术文献出版社重庆分社, 1978, 8: 11~14.
[11] 杨美珠, 陈右人. 茶树自交不亲和现象之观察[J]. 中国园艺, 2000, 46: 83~92.
[12] 陈迪新, 张绍铃, 陶书田. 沙梨花粉原位萌发与花粉管生长特性[J]. 南京农业大学学报, 2004, 27(3): 34~37.
[13] 李晓, 张绍铃, 陶书田, 等. 中国甜樱桃花粉原位萌发及花粉管生长的差异[J]. 西北植物学报, 2007, 27(3): 429~434.
[14] 薛妙男, 陈腾土, 杨继华. 沙田柚自交和异交亲和性观察[J]. 园艺学报, 1995, 22(2): 127~132.
[15] McClure BA, Haring V, R.Ebert P, et al. Style self-incompatibility gene products of Nicotiana alata are ribonucleases[J]. Nature, 1989, 342: 955~957.
[16] Lee H-S, Huang S, Kao T-H.S proteins control rejection of incompatible pollen in Petunia inflata[J]. Nature, 1994, 367: 560~563.
[17] Murfett J, Atherton TL, Mou B, et al. S-Rnase expressed in transgenic Nicotiana causes S-allele specific pollen rejection[J]. Nature, 1994, 367: 563~566.
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