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A Sequence Analysis and Structural Prediction on Polyphenol Oxidase in Tea

  • PAN Yu ,
  • WANG Kun-bo ,
  • XU Zhong-xi ,
  • XU Hui ,
  • HE Zhi-dan
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  • 1. The Key Laboratory of Tea Science of National Ministry of Education, Hunan Agricultural University
    2. Horticulture and Landscape College, Hunan Agricultural University
    3. Biological Sciences & Technology College, Hunan Agricultural University, Changsha 410128, China

Received date: 2007-11-26

  Revised date: 2008-03-06

  Online published: 2019-09-16

Abstract

In the present study, Polyphenol Oxidase (PPO) in tea plant which was registered in biodatabase (DDBJ & Uni-Prot) were analyzed and predicted by method of bioinformatics, including composition of nuclei acid sequences and amino acid sequences, signal peptide, transmembrane topological structure, hydrophobicity or hydrophilicity, secondary and tertiary structure of protein. The results showed that PPO in tea was a hydrophilic and non-transmembrane protein without signal peptide, but had coils. The main motifs of its predicted secondary structure of had random coil, alpha helix and beta turn were spread to the whole secondary structure of protein. Its amino acid sequences included two functional domains.

Cite this article

PAN Yu , WANG Kun-bo , XU Zhong-xi , XU Hui , HE Zhi-dan . A Sequence Analysis and Structural Prediction on Polyphenol Oxidase in Tea[J]. Journal of Tea Science, 2008 , 28(3) : 157 -165 . DOI: 10.13305/j.cnki.jts.2008.03.003

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