The phosphorus-releasing and potassium-releasing ability of two species of bacterium in soils of tea garden (pH5.5~6.5) was investigated under laboratory condition. The amounts of bacterium per unit volume were ascertained by the absorbance of culture solution under special wavelength and various pH. Results showed the absorbance was relatively high under pH5.5~6.5, that meant these bacterium grew well under the above pH range: the bacterial bio-fertilizer made of bacterial fermentation liquid and rapeseed cake can enhance the contents of fast-acting phosphorus and potassium, improve the growth of tea shoots and increase the tea yield after applied in the soils of tea garden. Results indicated that the selected bacterial strain and the produced bio-fertilizer showed good effectiveness in proving the soil fertility and growth of tea plant and increase the yield of tea garden.