The stability of the recombinant plasmid pET32a-GGT in the genetically engineered E. coli bacteria strain for the biosynthesis of theanine was in investigated this article. Result showed that the recombinant plasmid didn’t appear obvious gene deletion, showed structural stability after 100 generations. However, the plasmid is showed segregational instability, the plasmid-free cells appeared after 20 generations, and 18% cells was plasmid-harboring cells after 100 generation under no Amp selection pressure. It was structural and segregational stability under optimizing culture medium and condition during the fermentation process, with activity ofγ-GGT 4.64 U/ml and yield of theanine 35.18 g/L.
LU Wen-yuan
,
CHENG Hao
,
WANG Li-yuan
,
ZHOU Jian
. Study on the Plasmid Stability of the Genetically Engineered Escherichia coli Bacteria Strain for the Biosynthesis of Theanine[J]. Journal of Tea Science, 2008
, 28(2)
: 147
-151
.
DOI: 10.13305/j.cnki.jts.2008.02.001
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