Retention characteristics of theanine were investigated on C18 column for preparative high performance liquid chromatography (prep-HPLC). Based on frontal analysis and adsorption isotherm, effects of chromatographic conditions to separation were studied on overload injection. Results showed that the separation effect of volume overload is better than that of concentration overload. And large granule solid phase is recommended for large-scale purification of theanine on C18 column.
ZHANG Ying
,
SHI Zhao-peng
. Theanine Preparation with Preparative HPLC[J]. Journal of Tea Science, 2004
, 24(4)
: 283
-287
.
DOI: 10.13305/j.cnki.jts.2004.04.011
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