By using the methods of field investigation, microbial cultivation, pathological anatomy and electron probe, a preliminary investigation on the symptom, characteristic of occurrence and field distribution, etiology of the fluorescent green spot disease on tea leaves was conducted by the authors. Results showed that the representative symptom of this disease was green spots with fluorescence and irregular in shape; the lower epidermis of diseased leaves showed local convex which caused by the pathological changes of the spongy cells including the structural damage, volume expanding and the extrusion between the spongy cells. The occurrence of disease was closely related with the growth vigour of tea plant and nutrient supply. The distribution of disease in tea garden was characteristic of scattering and asymmetric, and having no disease center of occurrence. Pathogenic microbes weren’t discovered in the process of microbial isolation and cultivation. However, by transmission electron microscope, it could be observed that the cell membranes were damaged and many vacuoles formed in cytoplasm. By scanning electron microscope, various abnormal cells were found in tea foliage at the granular protrusions. Meanwhile, a certain amounts of calcium oxalate crystals were found within the spongy cells. On the basis of all above results, it could be deduced that this disease was classified to the physiological types．