Authors studied the influence of neem to acoustic communication of Empoasca Vitis by using the feeble sound monitoring system. The results indicated that, after feeding the tea shoots that systemic treated by neem seed extracts (NSE), the sound of male Empoasca vitis and sexes’ mating behavior were all affected. The higher the concentration of NSE, the bigger the influence was. The courtship sounds between the sexes that induced by male’ abnormal sound were very feeble, and the times using for mate meeting prolonged, their mating behavior lasted for shorter time. After spraying tea shoots with NSE, the acoustic communication and mating behavior of Empoasca vitis were not affected.