The effect of different hormone on proliferation and growth of tea tissue culture seedlings were investigated, and an optimal culture medium of MS + BA 2.0 + NAA 0.1 + GA3 3.0βmg/L was screened out. On this optimal medium, the proliferation rate of tea cultured buds was 2.75 times after 30’s incubation. And more than 20% of those buds could developed to seedlings higher than 5βcm.
ZHOU Jian
,
CHENG Hao
,
WANG Li-yuan
. The Optimization Research on Tissue Culture and Rapid Propagation of Camellia Sinensis[J]. Journal of Tea Science, 2005
, 25(3)
: 172
-176
.
DOI: 10.13305/j.cnki.jts.2005.03.003
[1] 严学成, 丘安经, 王毅军. 茶树叶片培养的器官发生[J]. 中药新药与临床生理, 1994, 5(2): 48~49.
[2] 刘德华. 茶籽子叶柄下胚轴组织培养的研究[J]. 茶叶通讯, 1990(3): 6~11.
[3] 王立, 杨素娟, 王玉书, 等. 茶种质资源室内保存技术研究—茶树茎切段试管苗保存技术[J]. 茶叶科学, 1996, 16(1): 37~42.
[4] 刘德华, 廖利民, 周带娣. 茶树组织培养的研究Ⅱ.腋芽微繁殖和叶微繁殖技术的研究[J]. 湖南农学院学报, 1991(增刊): 589~599.
[5] 郭玉琼, 赖钟雄, 郭志雄. 茶树生物技术研究进展[J]. 福建农林大学学报(自然科学版), 2002, 31(4): 470~475.
[6] 张文彤. 世界优秀统计工具—SPSS 11统计分析教程(基础篇)[M], 北京希望电子出版社, 2002, 6.
[7] Indra Sandal, Amita Bhattacharya, Paramvir Singh Ahuja.An efficient liquid culture system for tea shoot proliferation. Plant Cell, Tissue and Organ Culture, 2001, 65: 75~80.
[8] Mondal TK.Micropropagation of tea (Camellia sinensis). In: Jain SM & Ishii K (eds) Micropropagation of Woody Trees and Fruits. Kluwer Academic Publishers[M], The Netherlands.. 2003: 671~720.
浙公网安备 33019902000101号