The preparation of 14C labeled catechins was investigated by feeding 14C labeled precursor to tea cultured cells. Two labeled precursors were tested and their effects were compared. The dynamics of absorption and transformation of fed precursors and the changes of product composition and content caused by exogenous precursor were analyzed. Product analysis was also made by HSCCC and HPLC techniques.
CHENG Hao
,
LI Su-fang2
,
SHEN Xing-rong
,
SUN Jin-he
. Biosynthesis of 14C-catechins by Tea Cell Culture[J]. Journal of Tea Science, 2003
, 23(s1)
: 82
-87
.
DOI: 10.13305/j.cnki.jts.2003.s1.016
[1] Siro I Trevisanato, Young-In Kim.Tea and Health[J]. Nutrition reviews. 2000, 58(1): 1~10.
[2] 陈宗懋. 茶叶抗癌的生物化学基础[J]. 中国肿瘤. 2000, 9(1): 23~25.
[3] Cheng Hao, Yang Shujuan, Wang Yushu, et al. Studies on Catechin Accumulation in Tea Cultured Cells[A]. Proc of ’95 International Tea-Quality-Human health Symposium[C]. Nov 7-10, 1995 Shanghai, 1995: 308~312.
[4] 成浩,王玉书,杨素娟,等. 大量元素对茶愈伤组织生长及其儿茶素累积的影响[J]. 茶叶科学. 1994, 14(1): 31~36.
[5] 商业部茶叶畜产局,等. 茶叶品质理化分析[M]. 上海:上海科学技术出版社. 1989.
[6] Stafford H A, Shimamoto M, Lester H H.Incorporation of 14C phenylalanine into flavan-3-ols and procyanidins in cell suspension cultures of Douglas Fir[J]. Plant Physiol, 1982, 69: 1055~1059.
[7] Krisa S, Teguo P W, Decendit A, et al. Production of 13C-labelled anthocyanins by Vitis vinifera cell suspension cultures[J]. Phytochemistry, 1999, 51: 651~656.
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