RAPD markers analysis on the genome DNA of two sexual cultivars of Yunnadaye and Nankunshan maoyecha were studied by amplifying with 36 arbitrary 10-mer primers in order to defect the genetic diversity and relationship. Among the two sexual tea cultivars, 4382 loci were amplified, 109.55 loci per cultivars. In the total 315 DNA bands, 294 bands were polymorphic, the genetic diversity was 93.33%. It has been proved that Yunnadaye possess much higher genetic diversity than Nankunshan maoyecha on DNA molecular level. According to the cluster analysis, the two sexual cultivars might be divided into two groups by UPGMA method. 20 plants of Yunnadaye were classified as one group and 20 plants of Nankunshan maoyecha were classified as another group.
ZHOU Ying
,
DENG Pei-qing
,
YANG Hong-wei
,
LI Bin
,
YIN Yi
. Genetic Diversity of Two Sexual Tea Cultivars Detected by RAPD Markers[J]. Journal of Tea Science, 2003
, 23(2)
: 146
-150
.
DOI: 10.13305/j.cnki.jts.2003.02.012
[1] 张宏达. 山茶属植物的系统研究[J]. 中山大学学报, 1981, 20(1): 122~123.
[2] 张宏达, 叶创兴, 张润梅, 等. 中国发现新的茶叶资源––可可茶[J]. 中山大学学报. 1988, 27(3):131~133.
[3] 李斌. 南昆山毛叶茶染色体组型分析[J]. 中国茶叶,1995, (6): 30~31.
[4] 李斌, 陈娟, 郑永球, 等. 天然无咖啡碱茶叶资源的开发利用研究[J]. 食品科学, 2001, 22(7): 33~35.
[5] 谢冰芬, 刘宗潮, 王理开, 等. 毛叶茶和龙井茶提取物的抗瘤作用以及对DNA拓扑异构酶II的抑制作用[J]. 癌症, 1992, 11(6): 424~428.
[6] 宗潮, 谢冰芬, 潘启超. 毛叶茶提取物对不同细胞生长的影响及体内协同抗瘤作用[J]. 癌症, 1996, 15(3): 164~167.
[7] 陈亮, 陈大明, 高其康, 等. 茶树基因组DNA的提纯与鉴定[J]. 茶叶科学, 1997, 17(2): 177~181.
[8] Wachira F N, Powell W, Waugh R.An assessment of genetic diversity among Camellia sinensis L.(cultivated tea) and its wild relatives based on randomly amplified polymorphic DNA and organelle-specific STS[J]. Heredity, 1997, 78: 603~611.
[9] 陈亮, 杨亚军, 等. 15个茶树品种遗传多样性RAPD分析[J]. 茶叶科学, 1998, 18(1): 21~27.
[10] 李斌, 陈兴琰, 陈国本, 等. 茶树染色体组型分析[J]. 茶叶科学, 1986, 6(2): 7~14.