Isolation and Identification of Pathogens Causing Tea Gray Blight in Xishuangbanna Tea Area

ZHU Luojuan; JIANG Bingbing; MA Yuqing; BI Lingfang; HOU Guoxi; LI Rui; LIU Di; DUAN Siyan; ZHANG Jin; ZHU Shusheng; YANG Min; GUO Cunwu

doi:10.13305/j.cnki.jts.20251103.001

Journal of Tea Science >

2025 , Vol. 45 >Issue 6: 1047 - 1054

DOI: https://doi.org/10.13305/j.cnki.jts.20251103.001

Research Paper

Isolation and Identification of Pathogens Causing Tea Gray Blight in Xishuangbanna Tea Area

ZHU Luojuan ,
JIANG Bingbing ,
MA Yuqing ,
BI Lingfang ,
HOU Guoxi ,
LI Rui ,
LIU Di ,
DUAN Siyan ,
ZHANG Jin ,
ZHU Shusheng ,
YANG Min ,
GUO Cunwu

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1. National Engineering Research Center for Applied Technology of Agricultural Biodiversity, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China;
2. College of Plant Protection, Yunnan Agricultural University, Kunming 650201, China;
3. College of Tea Science, Yunnan Agricultural University, Kunming 650201, China;
4. Tea Research Institute, Yunnan Academy of Agricultural Sciences, Kunming 650205, China

Received date: 2025-06-09

Online published: 2025-12-10

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Abstract

Tea gray blight is one of the major foliar diseases in tea plantations, significantly affecting both yield and quality. In July 2023, tea leaves exhibiting resembling gray blight symptoms were observed during a filed surver in tea plantations in Mengla County, Xishuangbanna Dai Autonomous Prefecture, Yunnan Province. As the causal pathogen of these symptoms had not been previously reported in this region, this study aimed to identify the causal agent and its taxonomic classification. The pathogen was successfully isolated and identified using a combination of tissue isolation, pathogenicity testing via wound inoculation, morphological characterization, and molecular phylogenetic analysis based on ITS and TUB genes squences. The results confirmed that the strain FN3-2, isolated from symptomatic leaves, was pathogenic. Based on morphological traits and molecular evidence, the pathogen was finally identified as Neopestalotiopsis protearum. The finding provided a scientific basis for the accurate disease diagnosis and biocontrol agent screening, contributing to the sustainable development of tea production in the region.

Key words： Neopestalotiopsis protearum; gray blight; pathogen identification; tea

Cite this article

ZHU Luojuan , JIANG Bingbing , MA Yuqing , BI Lingfang , HOU Guoxi , LI Rui , LIU Di , DUAN Siyan , ZHANG Jin , ZHU Shusheng , YANG Min , GUO Cunwu . Isolation and Identification of Pathogens Causing Tea Gray Blight in Xishuangbanna Tea Area[J]. Journal of Tea Science, 2025 , 45(6) : 1047 -1054 . DOI: 10.13305/j.cnki.jts.20251103.001

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