Research Paper

Identification of A Pathogen Isolated from Tea Grey Blight Leaves

  • LIU Rong ,
  • YAO Yuxian ,
  • WANG Xing′e ,
  • LIU Bingxiang ,
  • SHUAI Qin ,
  • ZHAO Yongtian
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  • School of Life Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, China

Received date: 2025-10-23

  Revised date: 2025-12-27

  Online published: 2026-04-22

Abstract

Tea grey blight is one of the most important diseases in tea producing areas in China, which is common in old leaves, mature leaves and young bud leaves. In this study, a strain L352 was isolated and purified from tea grey blight leaves in Duyun City, Guizhou. The strain L352 was identified based on morphological characteristics and a multi-gene phylogenetic tree was established using the ITS, β-tub and EF-1α genes. The results show that the conidia of strain L352 were mostly composed of 5 cells with 4 transverse-septa, featuring three pigmented cells in the middle. Occasionally, 6 cells with 5 transverse-septa were observed, containing four pigmented cells in the middle. The phylogenetic tree indicates that it was clustered into a relatively independent clade with Pestalotiopsis rosea, with a support rate of 100%. The pathogen was finally identified as Pestalotiopsis rosea, and it was the first discovery of this species causing tea grey blight disease in Camellia sinensis.

Cite this article

LIU Rong , YAO Yuxian , WANG Xing′e , LIU Bingxiang , SHUAI Qin , ZHAO Yongtian . Identification of A Pathogen Isolated from Tea Grey Blight Leaves[J]. Journal of Tea Science, 2026 , 46(2) : 311 -317 . DOI: 10.13305/j.cnki.jts.2026.02.001

References

[1] 李冬雪, 赵晓珍, 王勇, 等. 贵州惠水县茶轮斑病病原菌的鉴定[J]. 热带作物学报, 2018, 39(9): 1827-1833.
Li D X, Zhao X Z, Wang Y, et al.Identification of the pathogen causing tea gray blight disease in Huishui County, Guizhou Province[J]. Chinese Journal of Tropical Crops, 2018, 39(9): 1827-1833.
[2] 陈文龙, 金道超, 李子忠. 茶树重要病虫害防治技术[M]. 贵阳: 贵州大学出版社, 2014.
Chen W L, Jin D C, Li Z Z, et al.Prevention and control technologies for major tea pests and diseases [M]. Guiyang: Guizhou University Press, 2014.
[3] Sanjay R, Ponmurugan P, Baby U I.Evaluation of fungicides and biocontrol agents against grey blight disease of tea in the field[J]. Crop Protection, 2008, 27(3/4/5): 689-694.
[4] Maharachchikumbura S S N, Guo L D, Liu Z Y, et al. Pseudopestalotiopsis ignota and Ps. camelliae spp. nov. associated with grey blight disease of tea in China[J]. Mycological Progress, 2016, 15(3): 15-22.
[5] Zhang Y M, Maharachchikumbura S S N, Wei J G, et al. Pestalotiopsis camelliae, a new species associated with grey blight of Camellia japonica in China[J]. Sydowia, 2012, 64(2): 335-344.
[6] 刘荣, 姚玉仙, 王兴娥, 等. 茶轮斑病的识别鉴定和防治的初步研究[J]. 农业与技术, 2025, 45(3): 69-72.
Liu R, Yao Y X, Wang X E, et al.Preliminary study on identification, identification and control of tea gray blight disease[J]. Agriculture and Technology, 2025, 45(3): 69-72.
[7] 孙晓玲. 中国重要茶树叶部病害的研究现状及展望[J]. 中国茶叶, 2016, 38(12): 12-13, 15.
Sun X L.Research status and prospects of important tea leaf diseases in China[J]. China Tea, 2016, 38(12): 12-13, 15.
[8] 李庆雨. 茶树轮斑病病原菌的鉴定及其对3种杀菌剂的敏感性[D]. 合肥: 安徽农业大学, 2022.
Li Q Y.Identification of pathogen causing gray blight on Camellia sinensis and their sensitivity to three fungicides [D]. Hefei: Anhui Agricultural University, 2022.
[9] Chen Y J, Zeng L, Shu N, et al.Pestalotiopsis-like species causing grey blight disease on Camellia sinensis in China[J]. Plant Disease, 2018, 102(1): 98-106.
[10] 谭琳, 谭济才. 茶树病虫防治学[M]. 3版. 北京: 中国农业出版社, 2021.
Tan L, Tan J C.Prevention and control of tea pests and diseases [M]. 3rd ed. Beijing: China Agriculture Press, 2021.
[11] 宋利沙. 拟盘多毛孢属的分类及分子系统学和分子条形码研究[D]. 南宁: 广西大学, 2016.
Song L S.Research on taxonomy, molecular systematics and DNA barcoding of Pestalotiopsis [D]. Nanning: Guangxi University, 2016.
[12] Jeewon R, Liew E C Y, Simpson J A, et al. Phylogenetic significance of morphological characters in the taxonomy of Pestalotiopsis species[J]. Molecular Phylogenetics and Evolution, 2003, 27: 372-383. doi: 10.1016/S1055-7903(03)00010-1.
[13] Jeewon R, Liew E C Y, HydeK D. Phylogenetic evaluation of species nomenclature of Pestalotiopsis in relation to host association[J]. Fungal Diversity, 2004, 17: 39-55. doi: 10.1016/S1055-7903(03)00010-1.
[14] 韦继光, 徐同, 郭良栋, 等. 从分子系统学角度分析拟盘多毛孢属及其近缘属的界限[J]. 广西农业生物科学, 2005, 24(3): 1-6.
Wei J G, Xu T, Guo L D, et al.Delimitation between Pestalotiopsis and allied genera based on result of molecular phylogenetics[J]. Journal of Guangxi Agriculture.and Biology Science, 2005, 24(3): 1-6.
[15] 刘爱荣. 海南植物内生拟盘多毛孢的多样性及拟盘多毛孢属分子系统学研究[D]. 杭州: 浙江大学, 2006.
Liu A R.Diversity of plant endophytic Pestalotiopsis in Hainan and molecular phylogenetics of Pestalotiopsis [D]. Hangzhou: Zhejiang University, 2006.
[16] 张维珊, 范鑫磊. 探究中国盘多毛孢真菌的物种多样性[C]//中国菌物学会. 中国菌物学会2024年学术年会论文摘要. 北京: 北京林业大学林学院, 2024: 350.
Zhang W S, Fan X L.Pestalotia sinensis explore the species diversity of Chinese Pestalotia sinensis fungi[C]//Mycological Society of China. Abstract of the 2024 Academic Annual Meeting of Mycological Society of China. Beijing: The College of Forestry of Beijing Forestry University, 2024: 350.
[17] Maharachchikumbura S S N, Guo L D, Chukeatirote, et al. Pestalotiopsis—morphology, phylogeny, biochemistry and diversity[J]. Fungal Diversity, 2011, 50(1): 167-187.
[18] 宋玉. 拟盘多毛孢属(Pestalotiopsis)真菌系统学研究及条形码基因的评价[D]. 贵阳: 贵州大学, 2015.
Song Y.Taxonomy of the genus Pestalotiopsis and evaluation of its DNA barcoding [D]. Guiyang: Guizhou University, 2015.
[19] 李应祥, 王勇. 贵州省都匀市茶轮斑病病原菌鉴定及生物学特性的研究[J]. 中国茶叶加工, 2013(3): 37-40.
Li Y X, Wang Y.The identification of tea gray blight pathogen and study of biological characters in Duyun City, Guizhou Province[J]. China Tea Processing, 2013(3): 37-40.
[20] Chen Y J, Zeng L, Shu N, et al.First report of Pestalotiopsis camelliae causing grey blight disease on Camellia sinensis in China[J]. Plant Disease, 2017, 101(6): 1034. doi: 10.1094/PDIS-01-17-0033-PDN.
[21] 陈雅丽, 陈文艳, 侯囡嵩, 等. 安徽池州市茶轮斑病病原鉴定及4种杀菌剂对其抑制性[J]. 安徽农业大学学报, 2019, 46(2): 209-213.
Chen Y L, Chen W Y, Hou N S, et al.Identification and inhibitory effect of four fungicides for pathogen causing tea gray blight in Chizhou, Anhui Province[J]. Journal of Anhui Agricultural University, 2019, 46(2): 209-213.
[22] 陈立杰, 宋玉, 陈通政, 等. 一个分离自贵州省茶叶上的拟盘多毛孢属新种[J]. 种子, 2019, 38(8): 70-73.
Chen L J, Song Y, Chen T Z, et al.One new Pestalotiopsis species isolated from Camellia sinensis of Guizhou Province[J]. Seed, 2019, 38(8): 70-73.
[23] 曹莹, 张爽, 齐鹰博, 等. 拟盘多毛孢属真菌侵染蓝莓的一个新纪录种: Pestalotiopsis kenyana[J]. 中国南方果树, 2023, 52(3): 138-143.
Cao Y, Zhang S, Qi Y B, et al.A new record of Pestalotiopsis spp. infection of blueberry: Pestalotiopsis kenyana[J]. South China Fruits, 2023, 52(3): 138-143.
[24] 韩凤英, 亓玉昆, 杨慧, 等. 松树赤枯病玫瑰拟盘多毛孢菌的鉴定及生物学特性研究[J]. 山东农业科学, 2024, 56(6): 113-119.
Han F Y, Qi Y K, Yang H, et al.Identification and biological characteristics of pestalotiopsis rosea causing pine needle blight[J]. Shandong Agricultural Sciences, 2024, 56(6): 113-119.
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