Based on the successful establishment of hyperuricemia model mice by potassium oxonate, black tea and its flower black brick tea made from the same fresh leaves were used to investigate their effects on lowering uric acid in hyperuricemic mice. In the study, ninety KM male mice were randomly divided into nine groups (10 mices for each group) including the normal group, the model group, the black tea-treated groups (high dose, middle dose, low dose) and the allopurinol group. Then all mice were orally administrated for fourteen days. The results showed that compared with the model group, the levels of weight , kidney index and serum uric acid in tea-treated groups were significantly decreased (P＜0.05). Moreover, the high-dose group of flower black brick tea had extremely significant reduction in blood uric acid (P<0.01). Blood urea nitrogen and serum creatinine also decreased significantly in tea-treated groups (P<0.01). The levels of urine urea nitrogen in the middle and high dose groups increased significantly (P<0.01), and the levels of urine uric acid in the high dose groups increased significantly (P<0.01). The levels of urine creatinine were significantly increased in the tea-treated groups (P<0.01) except the low-dose black tea group which showed an opposite trend (P<0.05). The middle dose group of flower black brick tea significantly decreased the activities of xanthine oxidase and adenosine deaminase (P<0.05). The middle dose group of black tea and both high dose groups all highly significantly reduced the activities of these two enzymes (P<0.01). These results showed that black tea and flower black brick tea could reduce the production of uric acid, and the high-dose flower black brick tea group was superior to black tea in lowering blood uric acid and increasing uric acid, urine urea nitrogen, and urine creatinine. The mechanism remains to be further explored.
YUAN Dongyin
,
XIAO Wenjun
,
PENG Yingqi
,
LIN Ling
,
ZHOU Yang
,
TAN Chunbo
,
TAN Hongbo
,
GONG Zhihua
. Effects of Black Tea and Its Flower Brick Tea on Lowering Uric Acid in Hyperuricemic Mice[J]. Journal of Tea Science, 2019
, 39(1)
: 34
-42
.
DOI: 10.13305/j.cnki.jts.2019.01.004
[1] 臧路平, 志刚, 吴新荣. 高尿酸血症发病机制及其药物治疗研究进展[J]. 医药导报, 2011, 30(1): 69-73.
[2] 杨会军, 李兆福, 万春平, 等. 尿酸盐转运蛋白在原发性通风及高尿酸血症中的研究进展[J]. 中华中医药学刊, 2013, 9(31): 1891-1893.
[3] 姚芳芳, 张锐, 傅锐娟, 等. 槲皮素对高尿酸血症大鼠黄嘌呤氧化酶和腺苷脱氨酶活性的影响[J]. 2011, 46(2): 248-250.
[4] Kuwabara M.Hyperuricemia, cardiovascular disease, and hypertension[J]. Pulse (Basel), 2016, 3(3/4): 52-242.
[5] 陈金华, 谭斌, 龚雨顺, 等. 红茶对高脂饮食小鼠血脂的调节作用[J]. 茶叶科学, 2015, 35(4): 384-396.
[6] 朱创, 刘增辉, 赵永青, 等. 红茶提取物对高尿酸血症小鼠血尿酸的影响[J]. 茶叶科学, 2017, 37(2): 167-172.
[7] Ali Alshatwi, Manal A Al Obaaid, Sahar A Al Sedairy, et al. Black and green tea improves lipid profile and lipid peroxidation parameters in Wistar rats feda high -cholesterol diet[J]. Physiol Biochem, 2011, 67(1): 95-104.
[8] Satoshi Uchiyama, Yoshimasa Taniguchi, Akiko Saka, et al.Prevention of diet-induced obesity by dietary black tea polyphenols extract in vitro and in vivo[J]. Nutrition, 2011, 27(3): 287-292.
[9] Anandhan A, Essa MM, Manivasagam T.Therapeutic attenuation of neuroinflammation and apoptosis by black tea theaflavin in chronic MPTP/probenecid model of Parkinson’s disease[J]. Neur Res, 2013, 23(2): 166-173.
[10] 王蝶, 黄建安, 叶小燕, 等. 茯砖茶减肥作用研究[J]. 茶叶科学, 2012, 32(1): 81-86.
[11] 傅冬和, 刘仲华, 黄建安, 等. 茯砖茶降脂功能成分研究[J]. 茶叶科学, 2012, 32(3): 217-223.
[12] 丁金祥, 黄昀, 李勇, 等. 茯砖茶抗氧化活性物质的提取工艺研究[J]. 食品研究与开发, 2013(16): 12-14,35.
[13] 余智勇, 黄建安, 杨明臻, 等. 茯砖茶抗腹泻效果研究[J]. 茶叶科学, 2009, 29(6): 465-469.
[14] 瞿绍明, 刘仲华, 刘杨. 茯砖茶与红茶对酒精性肝损伤的保护作用研究[J]. 食品与机械, 2013, 29(3): 4-7.
[15] Ran Zhou, Dong Chen, Hualing Wu.Pu-erh ripened tea resists to hyperuricemia through xanthine oxidase and renal urate transporters in hyperuricemic mice[J]. Journal of Functional Foods, 2017(29): 201-207.
[16] 彭雨轩, 熊哲, 胡志远, 等. 富金花茯散茶对高尿酸血症小鼠的影响[J]. 湖南城市学报, 2012, 4(21): 58-60.
[17] Chuang Zhu, Ling-Ling Tai, Xiao-chun Wan, et al. Comparative effects of green and black tea extracts on lowering serum uric acid in hyperuricemic mice[J]. Pharmaceutical Biology, 2017, 55(1): 2123-2128.
[18] 黄意欢. 茶学实验技术[M]. 北京: 中国农业出版社, 1995.
[19] 尉万春. “活血四妙汤” 对高尿酸血症的作用及其延缓尿酸性肾损害的机制研究[D]. 北京: 北京中医药大学, 2014.
[20] Johnson R J, Kang D H, Feig D, et al.Is there a pathogenetic role for uric acid in hypertension and cardiovascular and renal disease?[J]. Hypertension, 2003, 41(6): 1183-1190.
[21] 金红娜. 儿茶素类单体的降尿酸功效研究[D]. 沈阳: 沈阳农业大学, 2016.
[22] 欧阳梅, 熊昌云, 屠幼英, 等. 冠突散囊菌对茶叶品质成分及其抗氧化活性影响[J]. 菌物学报, 2011, 30(2): 343-348.
[23] Ginés S, Marino M, Mallol J, et al.Regulation of epithelial and lymphocyte cell adhesion by adenosine deaminase-CD26 interaction[J]. The Bionchemcal Journal, 2002, 361(Pt2): 203-209.
[24] 丁艳如, 陈爽, 朱忠顺, 等. 红茶菌的发酵条件及抗氧化活性[J]. 食品科技, 2016, 41(3): 21-25.
[25] 刘佳. 普洱茶多酚对高尿酸血症小鼠降尿酸和抗氧化作用的研究[D]. 昆明: 昆明理工大学, 2016.
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