By using Fuzhuan brick tea as raw material, five kinds of theabrownin with different molecular weight (≤3.5 kDa、3.5-25 kDa、25-50 kDa、50-100 kDa and ≥100 kDa) have been obtained by membrane separation technology. The spectral and physicochemical properties and inhibition on the α-glucosidase activity of theabrownins were comparative studied. The results showed 39 components from pyrolysis of Fuzhuan theabrownines were identified. The results showed that the theabrownins possessed the advantageous hypoglycemic effect. Effects of biological activity influenced by the total content of acidic group, carboxyl, phenolic hydroxyl, polysaccharide and protein. Five kinds of theabrownin belong to phenolic compounds containing hydroxyl, alkyl, carboxyl and phenyl, and showed a characteristic absorption peak at the 230 nm in UV-vis spectrum. The polysaccharide contents increased with the increase of theabrownin molecular weight, while the protein decreased. The theabrownin with molecular weight 25-50 kDa showed highest inhibition rate to α-glucosidase, which is equivalent of 1.9 times to that of acarbose. It is suggested that the Fuzhuan brick theabrownine is a benzene substance containing polyphenols, polysaccharides and proteins, which constitute a half chair or the chair conformation.
HE Guowen
,
ZHONG Tongsheng
,
PENG Xiaoyun
,
LONG Liping
,
ZHAO Yunlin
,
ZHANG Weimin
. Comparative Study on Component Characteristics and Inhibitory Activities on α-glucosidase of Theabrownin from Fuzhuan Brick Tea[J]. Journal of Tea Science, 2016
, 36(1)
: 102
-110
.
DOI: 10.13305/j.cnki.jts.2016.01.013
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