In order to protect the tea plant against freezing injury, the screening of antagonistic bacteria against INA bacteria No. 8 (Pantoea ananatis) and No. 14 (P. agglomerans) isolated from tea plant was carried out in Shandong Province. The strain Y1,an antagonistic bacteria against INA bacteria was isolated from the endophytic bacteria strains of tea plant. Based on the morphological characteristics and 16βS rDNA sequence analysis, the strain Y1 was identified as the Bacillus amyloliquefaciens. Through this study, the endophytic bacteria strain against the ice nucleation bacteria was obtained and identefied, it will be beneficial to the development of the biological control of ice nucleation bacterial.
HUANG Xiaoqin
,
ZHANG Lixia
,
LIU Huixiang
,
CHEN Zongmao
,
LI Duochuan
. Screeining and Identification of the Endophytic Bacterial Strain Against Ice Nucleation Active Bacteria of Tea Plant[J]. Journal of Tea Science, 2015
, 35(1)
: 97
-102
.
DOI: 10.13305/j.cnki.jts.2015.01.018
[1] 黄晓琴. 山东茶树冰核细菌的分离、鉴定及其与霜冻害关系研究[D]. 泰安: 山东农业大学, 2009: 50-55.
[2] 黄晓琴, 张丽霞, 刘会香, 等. 一株茶树冰核细菌拮抗菌的筛选及鉴定[J]. 茶叶科学, 2013, 33(6): 570-575.
[3] 王莉衡. 植物内生菌的研究进展[J]. 化学与生物工程, 2011, 28(3): 5-7.
[4] 程功, 张国财, 张欣倩, 等. 植物内生菌生物防治作用机制的探讨[J]. 中国林副特产, 2011(2): 77-80.
[5] 游见明. 茶树中内生菌的动态分布[J]. 广西植物, 2008, 28(1): 82-85.
[6] 陈晖奇. 茶树内生真菌的初步研究[D]. 福州: 福建农林大学, 2007: 26-28.
[7] 张婉婷, 张灵枝. 茶树内生真菌的分离和研究进展[J]. 中国茶叶, 2011, 33(6): 7-9.
[8] 洪永聪. 茶树内生枯草芽孢杆菌的研究[D]. 福州: 福建农林大学, 2006: 53-99.
[9] 陈百文. 茶树内生细菌对茶炭疽病的防病机制研究[D]. 福州: 福建师范大学, 2009: 18-43.
[10] 东秀珠, 蔡妙英. 常见细菌系统鉴定手册. 北京: 科学出版社, 2001: 267-295.
[11] R E布坎南, N E 吉本斯. 伯杰细菌鉴定手册[M]. 8版. 北京: 科学出版社, 1984: 751-752.
[12] 车晓曦, 李校堃. 解淀粉芽孢杆菌的研究进展[J]. 北京农业, 2010(1): 7-10.
[13] 周晨光, 徐圣君, 张茉莉, 等. 解淀粉芽孢杆菌微生物菌剂对茶叶产量和品质的影响[J]. 中国农学通报, 2014, 30(2): 253-257.