Tea plant (Camellia sinensis) is a hyper-accumulator of fluorine (F) and its leaves are the main accumulating tissue. In this paper, the F subcellular distribution and its binding characteristics with cell wall in tea leaves were investigated to explore the characteristics of F accumulating in tea leaves. The results showed that most of the F was distributed in the cell wall (39.74%-56.49%) and soluble fraction (28.35%-37.32%) of leaves. Pectin and hemicellulose are the main components of F enrichment and accumulated more than 90% of F in cell wall. There is a significant and positive correlation between Mn2+, Ca2+, Al3+ and F. The contents of F and metal elements (Mn2+, Fe3+, Ca2+, Mn2+, Al3+) reduced significantly in methylation and esterification cell wall while changed little in enzymolysis cell wall. These suggested that -NH2 and -COOH might play an important role in the combination of F and cell wall, while the molecule weights of pectin and cellulose played little role. Cell wall and soluble fraction are the main sites to store F where F might be combined with -OH, -NH2 and -COOH in the form of hydrogen bonds or with metal elements in the form of ionic bonds. F might be fixed in cell wall and vacuolar as the main defense mechanism of tea plant against F toxicity.
LIU Siyi
,
ZHU Xiaojing
,
FANG Fengxiang
,
ZHANG Haojie
,
QIU Andong
,
CHEN Yuqiong*
. Fluorine Subcellular Distribution and Its Combining Characteristics with Cell Wall in Tea Leaves (Camellia sinensis)[J]. Journal of Tea Science, 2018
, 38(3)
: 305
-312
.
DOI: 10.13305/j.cnki.jts.2018.03.011
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