New clonal tea varieties 0306D, 0306F, 0306I from F1 population of Baijiguan and Huangdan were employed as test materials. Antioxidant enzyme activities of tea plants under low temperature were measured. The results showed that leaves under low temperature had higher conductivity, malondialdehyde (MDA) and hydrogen peroxide (H2O2) contents with the increasing ratios higher in 0306D and 0306F than 0306I and Huangdan. The increasing ratios of peroxidase (POD), superoxide dismutase (SOD), ascorbate peroxidase (APX) and glutathione reductase (GR) activities of the Huangdan and 0306I tea varieties were higher than 0306D and 0306F. While monodehydroascorbate reductase (MDAR), dehydroascorbate reductase (DHAR), ascorbate (ASC), reduced glutathione (GSH) and catalase (CAT) activities of 0306D and 0306F were less decreased than 0306I and Huangdan. The results indicated that higher activities of defense enzymes and higher antioxidant content in 0306I and Huangdan under stress were associated with chilling tolerance. Further regression analysis showed that the H2O2 content was negatively correlated with APX and CAT, and conductivity was negatively correlated with ASC content. The above results indicated that the tolerance to low temperature in 0306I and Huangdan was stronger than 0306F and 0306D.
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